脾内转染白细胞介素-2和/或12基因增强接种肝癌大鼠T细胞功能研究

目的　探讨脾内直接注射白细胞介素IL 2、12基因对血IL 2和IL 12 ,以及T细胞活性的影响。方法　构建IL 2和或IL 12基因的逆转录病毒载体。含IL 2和或IL 12基因的包装细胞于不同时间进行脾内注射转染脾细胞。比较大鼠血IL 2和IL 12浓度、T细胞活性和毒性反应。结果　IL单基因治疗后血清IL 2或IL 12明显增高。IL 2 /IL 12联合基因组中血清IL 2和IL 12增加较单基因组显著。病理示肝癌组织中淋巴细胞浸润明显增多。IL治疗组治疗后 7d ,T细胞活性较对照组显著增高 (P <0 .0 5 )。联合基因组治疗后 7d ,T细胞功能较IL单基因组增强(P <0 .0 5 )。结论　脾内直接注射IL 2和或IL 12基因可增强T细胞活性 ,IL联合基因治疗优于IL单基因。

Enhancement of T cell activity by transfecting IL-2 and/or IL-12 genes into spleen in implanted liver cancer rats

Objective To study the effects of interleukin 12 (IL 12) and interleukin 2 (IL 2) genes injected intrasplenically on serum ILs and T cell activity in liver cancer.Methods The retroviral vectors encoding IL 2 and/or IL 12 genes were constructed.The packaging cell transfected by the vectors (IL genes) was injected directly into rat spleen at different time points.The serum IL 2 and IL 12,T cell activity and toxic effects were investigated.Results After treatment with IL alone for 7days,the serum IL 2 or IL 12 was increased markedly.In the group of combined use of IL 2/IL 12 genes,the levels of serum IL 2 and IL 12 were higher than those in group of IL alone.The pathological study showed that a large number of lymphocytes were infiltrated into liver tissues in the group of combined use of IL 2/IL 12 genes.As compared with control,T cell activity was increased markedly after treatment with IL for 7 days (P<0.05).After combined treatment with IL 2/ 12 gene for 7 days,T cells were activated significantly as compared with those in group of IL alone ( P <0.05).Conclusion IL 2 and/or IL 12 genes injected directly into spleen increase the T cell activity.Combined treatment with IL 2/IL 12 genes is superior to that of IL gene alone.