HPLC-ELSD法同时测定痔速宁片中鹅去氧胆酸、猪去氧胆酸的含量

目的 采用高效液相色谱法-蒸发光散射检测法测定痔速宁片中猪去氧胆酸和鹅去氧胆酸的含量。方法 采用Thermo U-3000液相色谱系统;ChromeleonTM 7工作站;ELSD 2000ES检测器,检测器条件：漂移管温度：105℃,撞击管关闭,增益为1,气体流速：2.0 L/min。采用Thermo（4.6×150 mm,C185μm 120 A）色谱柱;流动相：乙腈-0.1%冰醋酸（50∶50）;流速：0.9 mL/min;柱温：20℃。结果 表明猪去氧胆酸在（21.530～262.884）μg/mL（r=0.9997）,鹅去氧胆酸在（11.148～133.776）μg/mL（r=0.9995）的范围内呈良好的线性关系;猪去氧胆酸平均加样回收率为101.8%（n=9）,RSD=2.8%;鹅去氧胆酸平均加样回收率为98.0%（n=9）,RSD=2.3%。结论 本方法操作简便、准确、灵敏度高,可用于痔速宁片的质量控制。

Determination of hyodeoxycholic acid and chenodeoxycholic acid in zhisuning tablet by HPLC-ELSD

Objective To determine the hyodeoxycholic acid and chenodeoxyeholic acid in zhisuning tablet by HPLC- EISD. Methods Used Thermo U-3000 HPLC, ChromeleonTM 7 and ELSD 2000ES, drift tube temperature was 105℃, impactor was off, gain was 1, gas flow was 2.0 L/min;Thermo column （4.6x150 mm,C18 5 μm 120A） was used with the methanol-0.1%Glacial acetic acid （50：50） as mobile phase at a flow rate of 0.9 mL/min; The column temperature was 20℃. Results The linear range of hyodeoxyeholic acid was （21.530-262.884） μg/mL（r=0.9997）, the average recovery was 101.8%（n=9）, RSD=2.8%; The linear range of ehenodeoxycholic was （11.148-133.776） μg/mL（r=0.9995）, the aver- age recovery was 98.0%（n=9）, RSD=2.3%. Conclusion This method is simple, sensitive, and accurate for the quality control of astraloside in zhisuning tablet.