肿瘤相关成纤维细胞在垂体腺瘤侵袭性行为中的作用研究

目的 初步研究肿瘤相关成纤维细胞在垂体腺瘤侵袭性行为中的作用。方法 依据术前磁共振（MRI）检查和术中观察，收集典型侵袭性（IPA组，n=7）和非侵袭性（nIPA组,n=8）垂体腺瘤新鲜组织，进行原代培养肿瘤相关成纤维细胞，并用肌动蛋白α（α-SMA）染色鉴定；利用Transwell迁移和侵袭性试验比较两组细胞的迁移和侵袭能力，并用免疫细胞化学、Western blot比较两组肿瘤相关成纤维细胞α-SMA、基质金属蛋白酶（MMP）-9的表达差异。结果 新鲜垂体腺瘤组织中培养出α-SMA阳性的肿瘤相关成纤维细胞，两组细胞的迁移能力无明显差异，侵袭能力IPA组强于nIPA组（ P=0.010）；IPA组细胞MMP-9表达高于nIPA组（ P=0.025），α-SMA的表达在两组之间差异无统计学意义。结论 肿瘤相关成纤维细胞可能通过分泌更多的MMP-9增强自身侵袭能力，为肿瘤细胞侵袭周围组织提供条件。

The Role of Cancer-associated Fibroblasts in Invasive Behavior of Pituitary Adenoma

Objective To determine the role of cancer associated fibroblasts (CAFs) in the invasive behavior of pituitary adenoma. Methods Pituitary adenoma tissues were divided into invasive group (IPA) and non-invasive group (nIPA) according to pre-operative MRI and observations during surgery. Those tissues were cultured and CAFs were identified through α smooth muscle actin (α-SMA). The migratory and invasive ability of CAFs was tested with transwell migration and invasion assay. The expressions of α-SMA and matrix metalloproteinase (MMP)-9 from CAFs were determined by immunocytochemistry and Western blot. Results All cultured CAFs expressed α-SMA. No significant difference in migratory ability of CAFs was found between the IPA and nIPA tissues; however, CAFs from the IPA tissues had stronger invasive ability than those from the nIPA tissues ( P=0.010). Higher levels of MMP-9 expression were found in group IPA as compared with nIPA ( P=0.025). No significant difference in the expression of α-SMA was found between the two groups. Conclusion CAFs may promote invasive behavior by secreting more MMP-9, which may play a part in the invasive behavior of pituitary adenomas.