HCV抗体检测试剂对血站HCV抗体初筛试剂的评价

目的 建立对血站献血人员血液中HCV抗体初筛试剂的评价方法，为选择合适匹配的初筛试剂提供依据。方法 用新研制出的抗HCV不同基因功能区抗体检测试剂对两种国产试剂和一种进口试剂的特异性和灵敏度进行考核评价。结果 两种国产试剂之间的特异性和灵敏度均存在一定差异。且总体质量略差于进口试剂。结论 用新研制出的抗HCV不同基因功能区抗体检测试剂可对初筛试剂进行评价，选择合适的初筛试剂匹配对控制献血员的血液质量至关重要。

Evaluation of anti-HCV detection kits using recombinant antigens derived from various HCV regions

Objective To evaluate the first and second assay kits currently used in blood centers for screening HCV infected blood, and to provide basis for a better match of the two assay kits. Methods Using the newly developed multi-recombinant-HCV-antigen supplementary assay kit, the authors evaluated concurrently the specificity and sensitivity of two domestic and one imported anti-HCV detection kits. Results Discrepancy in specificity and sensitivity existed among the two domestic HCV kits, and overall quality was slightly below that of leading or main stream imported HCV kit.Conclusion The newly developed multi-recombinant-HCV-antigen supplementary assay kit is useful in the evaluation of HCV antibody detection kit curently in use. It provides qualified assessing kit to capture antibodies against various HCV antigens. The present paper provided guidence for selecting a better match of the two screennig kits and improved screening efficiency.