肝细胞癌组织中ZEB-1的表达及意义

目的 探讨ZEB-1在肝细胞癌(HCC)组织中的表达及意义.方法 采用免疫组织化学SP法和蛋白质印迹分析法检测ZEB-1在62例HCC组织、48例相应癌旁肝硬化组织和10例正常肝组织中的表达,并分析其与HCC临床病理特征的关系.结果 免疫组化结果显示ZEB-1在HCC组织及癌旁肝硬化组织中呈阳性表达,阳性率分别为93.5％(58/62)和83.3(40/48).蛋白质印迹分析显示ZEB-1蛋白在HCC组织中的表达高于相应癌旁肝硬化组织和正常肝组织(P＜0.05).ZEB-1在HCC组织中的表达与临床分期、肿瘤分化程度、有无发生转移、有无门脉癌栓及有无术后复发等因素相关(P＜0.05),与肿瘤直径、肿瘤数目、血清AFP水平等因素无关(P＞0.05).结论 ZEB-1的高表达与肝癌的发生、发展密切相关,并且有可能参与HCC细胞的上皮间质转化而促进肝癌的侵袭和转移.

Expression of ZEB-1 in hepatocellular carcinoma tissues and its significance

ObjectiveTo investigate ZEB-1 protein expression in hepatocellular carcinoma (HCC) tissues and the relevant significance. MethodsImmunohistochemical method and Western blotting analysis were used to detect the ZEB-1 protein expression in 62 HCC tissues,48 paracancerous cirrhotic tissues and 10 normal liver tissues. The relationship between ZEB-1 protein expression and clinicopathological features of HCC was evaluated. ResultsImmunohistochemical staining showed that the positive rates of ZEB-1 protein expression in HCC tissues and paracancerous cirrhotic tissues were 93.5% (58/62) and 83.3 (40/48), respectively. Western blotting analysis showed that ZEB-1 protein expression in HCC tissues was significantly higher than those in the paracancerous cirrhotic tissues and normal liver tissues (P<0.05). We also found that ZEB-1 expression was significantly correlated with the clinical stage, tumor differentiation degree, metastasis, portal vein tumor thrombus and postoperative recurrence (P<0.05), and was not correlated with the tumor diameter, number of tumors, or serum AFP levels (P>0.05). ConclusionHigh expression of ZEB-1 is correlated with the development and progression of HCC. ZEB-1 might also participate in the epithelial-mesenchymal transition of HCC cells, subsequently contributing to the invasion and metastasis.