OPG-HSP70融合蛋白对CIA大鼠的保护性作用

目的:研究人骨保护素(OPG)-分枝杆菌热休克蛋白70(HSP70)融合蛋白对类风湿关节炎动物模型--胶原诱导的关节炎(CIA)大鼠的保护性作用.方法:将OPG-HSP70重组基因菌株诱导表达,收集蛋白并鉴定,大量提取,纯化,复性;实验大鼠分为OPG-HSP70组、OPC组、HSP70组、模型组和正常对照组,CIA大鼠模型制备后分别给予相应处理,观察对大鼠关节病变的影响.结果:与模型组相比,OPG-HSP70组关节炎指数明显降低(P<0.05);关节病理切片显示关节滑膜无明显增生;骨密度值增高(P<0.05).结论:OPGHSP70融合蛋白具有减轻CIA大鼠关节病变的作用.

Protective effects of OPG-HSP70 fusion protein on CIA rats

AIM:To study the protective effects of human osteoprotegerin (OPG)-Mycobacterium tuberculosis heat shock protein 70 (HSP70) fusion protein on animal models of the rheumatoid arthritis by the observation of the symptoms of collagen-induced arthritis (CIA) rats.METHODS:The OPG-HSP70 recombinant strain stored in the laboratory were induced by isopropyl B-D-thiogaiactopyranoside (IPTG) and the acquired fusion protein were collected and identified,purificated and dialysis refolded.The experimental rats were divided into OPG-HSP70 group,OPG group,HSP70 group,model group and normal group,and were treated with corresponding protein after preparation.The symptoms and signs of collagen-induced arthritis rats were observed carefully.RESULTS:Compared with the positive control group,the OPG-HSP70 fusion protein group demonstrated that the arthritis index was reduced significantly (P<0.05);the clear structures of the intra-articular synovials and the bone mineral density were increased (P<0.05) from articular pathology studies.CONCLUSION:OPG-HSP70 fusion protein shows efficacy in alleviating the symptoms of CIA rats.