apoH基因多态性与脑卒中的相关性研究及其对血脂的影响

目的：探讨载脂蛋白H（apolipoprotein H,apoH）基因第8外显子G1025C（Try316Ser）多态性与长沙地区汉族人脑卒中的关系及其对血 脂的影响。方法：采用PCR－单链构象多态技术和DNA直接测序法检测长沙地区汉族260例脑卒中患者、20个脑卒中家系成员和100名健康对照者的apoH基因第8外显子G1025C（Try316Ser）多态性；酶联免疫吸附法检测抗磷脂抗体水平；酶法测定总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL－C）、低密度脂蛋白胆固醇（LDL－C），免疫比浊法测定apoA-I及apo B100，酶联免疫吸附法测定脂蛋白a血清水平。结果：长沙地区汉族人apoH基因G1025C（Try316Ser）多态存在GG、GC两种基因型，脑卒中组及其各亚组G1025C（Try316Ser）多态基因频率分布与对照组比较差异无显著性（P＞0．05）；分析不同基因型对血脂、脂蛋白水平的影响，发现脑梗塞组和对照组GC基因型血清TG水平均明显高于GG基因型（P＜0．05）。结论：长沙地区汉族人apoH基因G1025C（Try316Ser）多态性可能与脑卒中的易患性无关，但与血脂代谢有一定关联。

The relationship of apolipoprotein H G1025C(Try316Ser)polymorphism with stroke and its effect on plasma lipid levels in Changsha Hans

Objective To investigate the relationship between G1025C(Try316Ser)polymorphism in exon 8 of apolipoprotein H (apoH) gene and stroke and to evaluate the effect of G1025C(Try316Ser)polymorphism on plasma lipid levels in Changsha Hans. Methods G1025C(Try316Ser) polymorphism in apoH gene was determined by PCR single strand conformation polymorphism analysis and DNA sequencing in 100 healthy controls,260 patients with stroke, and 20 stroke pedigrees. Serum antiphospholipid antibody (APA) levels were tested by enzyme linked immunosorbent assay(ELISA). Plasma lipid levels were measured by routine methods. Results No statistically significant differences were found in frequencies of genotypes and alleles of G1025C(Try316Ser) polymorphism between the controls and stroke patients. The serum levels of TG in the GC genotype of cerebral infarction patients and controls were markedly higher than those in GG genotype. Conclusion There was no association between G1025C(Try316Ser)polymorphism and stroke in Changsha Hans. G1025C(Try316Ser) polymorphism was associated with plasma lipid metabolism in Changsha Hans.