大鼠局灶性脑缺血再灌注后凋亡诱导因子的表达与神经元凋亡的关系

Objective To study the relationship between the expression of apoptosis inducing factor (AIF) and neuronal apoptosis in rats with focal ischemia reperfusion.Methods Wistar rats were divided into sham-operated (n=6) and model (n=30) groups.Middle cerebral artery occlusion (MCAO)models were established by thread ligation.The expression of AIF in the ischemic penumbra was observed in the sham-operated and model groups 6 h and 1,2,3 and 7 d after the reperfusion; the changes of neuronal apoptosis in corresponding region were observed by TUNEL staining at the same time.Results AIF-positive cells in the ischemic penumbra were significantly increased in the model group 6 h after the ischemic reperfusion,reaching its peak level at 48 h of reperfusion (130.47±11.32 cells).Cell apoptosis occurred at each time point of ischemic reperfusion and the percentage of apoptosis cells at 48 h ofreperfusion (118.53±11.67 ceils) was the highest among all the time points.Significant differences between the 2 groups were found between each 2 time points (P<0.05).Conclusion Focal ischemia reperfusion can induce the increased expression of AIF positive cells and neuronal apoptosis.

Relationship between expression of apoptosis inducing factor and neuronal apoptosis in rats with focal cerebral ischemia