| RP-HPLC法测定芦荟中芦荟苷的含量 |
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| 引用本文： | 张永文,陈玉武,李克明.RP-HPLC法测定芦荟中芦荟苷的含量[J].医学教育探索,1999(8):577-578. |
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| 作者姓名： | 张永文 陈玉武 李克明 |
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| 作者单位： | 中日友好临床医学研究所!北京100029 |
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| 摘 要： | 应用反相ＨＰＬＣ法测定芦荟苷的含量。用ＳＵＰＥＬＣＯ ＬＣ－１８层析柱，流动相：乙腈－０．０１ｍｏｌ／Ｌ三氟醋酸（２２：７８），流速：１ｍＬ／ｍｉｎ检测波长；３５９ｎｍ，柱温：室温，用外标法测定。芦荟苷的平均回收率为９８．３７％，其检测线性范围为５０￣２５０μｇ／ｍＬ，回归方程Ｃ＝０．２６９＋５４．７２３Ｘ，ｒ＝０．９９９９本法灵敏，简便，重现性好。
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| 关 键 词： | 芦荟 芦荟苷 含量测定 RP－HPLC |
Determination of Aloin in Barbados Aloe (Aloe barbadensis) and Robust Aloe (Aloe ferox) by RP-HPLC |
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| Zhang Yongwen,Chen Yuwu,Li keming.Determination of Aloin in Barbados Aloe (Aloe barbadensis) and Robust Aloe (Aloe ferox) by RP-HPLC[J].Researches in Medical Education,1999(8):577-578. |
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| Authors: | Zhang Yongwen Chen Yuwu Li keming |
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| Abstract: | A RP-HPLC method was developed for the determination of aloin in Aloe barbadensis Mill.. and A. ferox Mill. An ODS column was used with the mobile phase of acetonitrile-0. 01 mol/L trifluoracetic acid (22: 78) at a flow rate of 1 mL/min. Samples were detected at 359 nm and at room temperature. External standard was used and the calibration curve was linear over the range of 50~ 250 ug/mL (r=0. 9999). The extraction recovery rate was 98. 37%. This method is sensitive, simple and well reproducible. |
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| Keywords: | Aloe barbadensis Mill Aloe ferox Mill RP-HPLC |
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