三七总皂苷对TNF、NO含量的影响及其机制研究

采用ｓｃ角叉菜胶２５ｍｇ／ｋｇ复制大鼠背部气囊滑膜炎模型。白细胞计数，蛋白含量分别用试管稀释法和Ｌｏｗｒｙ法测定。酶联免疫吸附分析法Ｒｉｖａｎｏｌ法和Ｆｕｒａ－２荧光分析法分别用于测定灌洗液中ＴＮＦ、ＮＯ含量以及中性粒细胞胞内游离钙水平的变化。

Effects of Total Saponins of Sanchi (Panax pseudo-ginseng var. notoginseng)on TNF, NO and Its Mechanisms

Rat air-pouch acute imflammatory model was prepared by sc injection of carrageenan (Car, 25 mg/kg). The neutrophil (Neu) count and protein content in exudate were measured by test tube diluting method and Lowry method. Enzyme linked immunosorbent assay and Rivanol assay were applied respectively to investigate effects of saponins of Panax pseudo-ginseng var. notoginseng (Burk.) Hoo et Tseng (PnS) on tumor necrosis factor (TNF) and nitric oxide (NO) content in the perfusate. Fura-2 fluorescence technique was used to determine the intracellular free calcium concentration in Neutrophils (Neu-Ca2 ]i). The results showed that PnS 60, 120 and 240 mg/kg ip reduced Neu counts, protein content, TNF and NO contents in a dose-dependent manner; lowered the level of Neu-Ca2 ], with inhibitory rates of 9. 2%, 34. 3%, and 39. 2%, respectively. It was concluded that PnS has an obvious antiinflammatory effect and its mechanisms of action are related to the reducton of TNF and NO contents, and inhibition of Neu-Ca2 ], level.