引滦水源隐孢子虫和f2噬菌体消长规律研究

目的 探讨天津引滦水源原虫与病毒污染现状和消长规律。方法 根据引滦河道的排污、与其他水体交叉及其倒虹闸的分布情况确定采样监测点。分别用隐孢子虫和f2噬菌体表示水体受原虫和病毒污染的情况，水样通过浓集分离装置使隐孢子虫和f2噬菌体得到浓集，隐孢子虫经过固定、洗脱、悬浮和洗涤、染色，镜检计数；f2噬菌体滤液中加入Ecoli 285寄主菌液，恒温培养，观察计数。结果 各采样点隐孢子虫和f2噬菌体点次检出率分别为57．7％和63，9％，分别集中在春夏季和秋季。f2噬菌体在中上游较高，于桥水库和下游较低。结论 引滦水源已受原虫和病毒的污染。建议将引滦河道中极易受到污染的自然河道和明渠段改为暗渠输水方式，并加强引滦河道沿岸工农业和生活污染来源的监控。

Study on dynamic changes of cryptosporidium and bacteriophage f2 in source water diverted from Luan river

Objective To grasp the pollution situation and dynamic changes of protozoan and viruses in the source water diverted from Luan River.Methods The sampling sites were choosed according to pollutants drained along the Luan River-diverted channel,the situation of crossing with other rivers and the distribution of water brakes.The pollution of source water polluted by protozoan and viruses were indicated respectively by cryptosporidium and bacteriophage f 2.They were condensed by special appliance.Cryptosporidium was counted under microscope after a serial of treatments.Put Ecoli 285 host into filtered water containing bacteriophage f 2,and cultivated homoiothermicly,then counted.Results The results were positive of each site,the point rates of cryptosporidium and bacteriophage f 2 were 57.7% and 63.9% respectively,and highly concentrated in spring-summer and autumn.The counts of cryptosporidium in the sites were high except Yuqiao Reservoir and Yixingbu Pump Station;the counts of bacteriophage f 2 were higher in the upper-end-middle stream,and lower at Yuqiao Reservoir and the downstream.Conclusion The source water diverted from Luan River was polluted by protozoan and viruses.It was suggested that the natural waterways and ditches uncovered should be covered,and surveillance should be imposed on pollution source along the channel.