基质金属蛋白酶-9及其抑制物-3在人种植窗期子宫内膜的表达特点及作用

目的　探讨基质金属蛋白酶 9(MMP 9)和组织金属蛋白酶抑制物 3(TIMP 3)蛋白在人种植窗期子宫内膜组织中表达的特点 ,及其在胚胎着床中的作用。方法　应用链菌生物素蛋白 过氧化酶连接法 ,观察 13例增殖期、10例分泌早期、17例种植窗期及 15例分泌晚期子宫内膜组织中MMP 9和TIMP 3蛋白的表达特点及其阳性细胞的分布规律。结果　MMP 9和TIMP 3蛋白在各期子宫内膜中均有表达 ,从增殖期到分泌早期至种植窗期 ,MMP 9和TIMP 3蛋白染色逐渐增强 ,以种植窗期增强最为明显 ,分泌晚期染色强度虽较种植窗期略低 ,但仍明显高于增殖期。结论　种植窗期子宫内膜中MMP 9和TIMP 3蛋白为高表达 ,可能参与胚胎着床的调节 ,有利于胎盘的形成

Expression of Matrix Metalloproteinase -9 and Tissue Inhibitor of Metalloproteinase -3 Protein in the Implantation Window Phase of Endometrium

Objective To study the expression of matrix metalloproteinase 9 (MMP 9) and tissue inhibitor of metalloproteinase 3 (TIMP 3) protein in the implantation window phase of endometrium and the role of MMP 9 and TIMP 3 on embryo implantation. Method Immunohistochemical analysis was employed to demonstrate the population of the MMP 9 and TIMP 3 positive cell in endometrium of different phases of menstrual cycle. Results The positive cells of these soluble proteinases were found to be located in the endometrial epithelial cells and stromal cells. The quantitative analysis of MMP 9 and TIMP 3 proteins demonstrated an increase in staining exent from proliferative endometrium to the endometrium of implatation window phase, and an decrease to late secretory endometrium in comparison with the implantation window phase. Conclusion Expression of MMP 9 and TIMP 3 in the implantation window phases of endometrium is specific and may play an important role in regulating the depth of trophoblast invasion during embryo implantation and may be involved in endometrial stromal breakdown during menstruation.