| HCV感染者血清及外周血单个核细胞HCV RNA检测及其意义 |
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| 引用本文： | 刘文恩,谭德明,范学工,张铮,甘晓明,欧阳颗,谢玉桃.HCV感染者血清及外周血单个核细胞HCV RNA检测及其意义[J].中国现代医学杂志,2001,11(2):31-32. |
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| 作者姓名： | 刘文恩 谭德明 范学工 张铮 甘晓明 欧阳颗 谢玉桃 |
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| 作者单位： | 中南大学附属湘雅医院传染科 |
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| 摘 要： | 目的：探讨同时检测HCV感染者血清及外周血单个核细胞（PBMC）中HCVＲＮＡ的意义。方法：应用ＲＴ－ＰＣＲ方法对３１例ＨＣＶ感染者进行血清及ＰＢＭＣ ＨＣＶ ＲＮＡ检测。结果 ＰＢＭＣ ＨＣＶRNA阳性率（74．19％，23／31）显著高于血清HCV RNA阳性率（23．03％，9／31）。结论：同时检测HCV感染者血清及外周血单个核细胞（PBMC）中HCV RNA可减少漏诊，PBMC中的HCV可能是HCV感染慢性化的重要之一。通过对HCV感染者同时进行血清及PBMC中HCV ＲＮＡ检测，可为丙型肝炎的诊断、治疗及预后的判断提供较为可靠的指标，对丙型肝炎慢性化机制的研究具有重要意义。
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| 关 键 词： | 单个核细胞 丙型肝炎病毒 聚合酶链反应 |
| 修稿时间： | 1999年8月16日 |
SIGNIFICANCE OF PERIPHERAL
BLOOD MONONUCLEAR CELL HC V RNA IN PATIENTS WITH HCV INFECTION |
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| Liu Wen-en,Tan Deming,Fan Xuegong,et al..SIGNIFICANCE OF PERIPHERAL
BLOOD MONONUCLEAR CELL HC V RNA IN PATIENTS WITH HCV INFECTION[J].China Journal of Modern Medicine,2001,11(2):31-32. |
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| Authors: | Liu Wen-en Tan Deming Fan Xuegong |
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| Institution: | Liu Wen-en,Tan Deming,Fan Xuegong,et al. Department of Infection Disease,Xiangya Hospital Zhongnan Medical University,Changsha,410008 |
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| Abstract: | To investigate the significance of HCV RNA detection in PBMC in patients with HCV infection.Methods:Al l of 31 sera and PBMC taken from HCV infection were used for HCV RNA detection by RT-PCR.Results:PBMC HCV RNA positive rate (74.19%,23/31)was sig ni ficant higher than those of sera HCV RNA (23.03%,9/31)(χ2＝11.770,P＞0.005).Conclusions:PBMC HCV may be one of important reasons for mechani sm of chronicity pathogenesis HCV infection |
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| Keywords: | Hepatitis C Virus Mononuclear Cell Polymerase Chain Resaction |
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