人类乳腺癌基因表达分析

目的研究乳腺癌发生和发展相关基因群的表达及其初步功能。方法用4096种人类基因多聚酶链反应(PcR)产物制成BioDoor4096型表达谱芯片，分离纯化正常乳腺组织和乳腺癌组织mRNA，制备表达谱探针，用ScanArray3000荧光扫描仪扫描芯片荧光信号图像，利用计算机分析正常乳腺组织和乳腺癌组织之间差异表达的基因。结果在4096种基因中，正常乳腺组织和乳腺癌组织之间差异表达的基因有619条(15．11％)。生物信息学分析显示，这些差异表达的基因可能与乳腺癌的发生和发展密切相关。结论乳腺癌的发生、发展中存在多基因表达调控的改变，对于相关基因群的研究有助于认识肿瘤发病机制，并为乳腺癌个体化治疗提供依据。

Differentially expressed genes in normal breast tissue and breast cancer tissue by cDNA microarray

Objective To screen for the differentially expressed genes in normal breast tissue and breast cancer tissue by using cDNA microarray.Methods The PCR products of 4096 genes were spotted on chemical material coated glass plates in array.The total RNAs were isolated from tumor tissue and normal tissue and were purified to mRNA by Oligotex.Both the mRNAs were reversely transcribed to cDNAs with the incorporations of fluorescent dUTP,for preparing the hybridization probes.The mixed probes were then hybridized to the cDNA microarray.Microarray was scanned for the fluorescent signals and showed the differences between the two samples.Results Among the 4096 target genes,there were 619 (15.11%) genes whose expression levels differed between normal breast tissue and breast cancer tissue.Bioinformational analysis of those genes had been performed.Conclusion DNA microarray technology is an effective technique in screening for differentially expressed genes between two different kinds of tissues.Further analysis of the obtained genes will help to understand the molecular mechanism of malignant carcinoma.