Protective Effect of Interleukin-lβ on Motor Neurons after Sciatic Nerve Injury in Rats |
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摘 要: |
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关 键 词: | 运动神经 坐骨神经损伤 白细胞介素-lβ 动物实验 神经生长因子 神经功能缺损 |
收稿时间: | 11 June 2002 |
Protective effect of interleukin-1β on motor neurons after sciatic nerve injury in rats |
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Authors: | Weng Yuxiong Khatri Bharat Hong Guangxiang Wang Fabin Chen Zhenbin Huang Qishun |
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Institution: | (1) Department of Hand Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022 Wuhan |
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Abstract: | Summary Protective effect of interleukin-1β (IL-1β) on motor neurons was studied after peripheral nerve injury. Twenty Wistar rats
were divided into 2 groups randomly. The right sciatic nerve of each rat was resected. After silicon tubulization of sciatic
nerve in rat, 15 μl 1 ng/ml IL-1β and PBS solution were injected into the silicon capsule respectively. Enzyme histochemistry
was performed to show acctyle cholesterase (AchE) and nitric oxide staining (NOS) activity of spinal α motor neurons in spinal
segments 2 weeks later. Neurons were counted and the diameter and cross sectional (c/s) area of neurons were analyzed by using
computer image analysis system. The results showed that as compared with the normal side, both enzyme activities significantly
changed in motor neurons in PBS group. The diameter and c/s area of both neurons changed significantly too (P<0.01). These results suggest that exogenous IL-1β protects α-motor neurons from degeneration and necrosis after peripheral
nerve injury.
WENG Yuxiong, male, born in 1971, Doctor in Charge |
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Keywords: | protective effect interleukin-1β motor neurons nerve injury |
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