| miR-17-92基因簇反义寡核苷酸对胃癌细胞增殖与侵袭的抑制作用 |
| |
| 引用本文： | 姚烜,张灵敏,汪洋,姚煜,纪宗正.miR-17-92基因簇反义寡核苷酸对胃癌细胞增殖与侵袭的抑制作用[J].陕西肿瘤医学,2011(5):852-855. |
| |
| 作者姓名： | 姚烜 张灵敏 汪洋 姚煜 纪宗正 |
| |
| 作者单位： | [1]西安交通大学医学院第二附属医院,陕西西安710004 [2]西安交通大学医学院第一附属医院,陕西西安710061 |
| |
| 基金项目： | 陕西省科技攻关基金资助项目（编号：2010kw-17） |
| |
| 摘 要： | 目的：探讨miR-17-92基因簇反义寡核苷酸对人胃癌细胞株SGC-7901的增殖及侵袭的影响。方法：合成miR-17-92基因簇中miR-17、miR-18a、miR-20a的反义寡核苷酸,通过实时荧光定量PCR验证miR-17、miR-18a、miR-20a反义寡核苷酸对SGC-7901细胞的miR-17、miR-18a、miR-20a的抑制效果,然后利用MTT、Transwell检测抑制表达后对细胞增殖及侵袭的影响。结果：miR-17、miR-18a、miR-20a的反义寡核苷酸能够抑制对应miRNA的表达,同时抑制了胃癌细胞的增殖与侵袭。结论：miR-17-92基因簇可能在胃癌发生发展中起到了癌基因的作用。
|
| 关 键 词： | miR-17-92基因簇 胃癌 SGC-7901 增殖 转移 |
Effect of miR - 17 -92 cluster on proliferation and metastasis of human gastric cancer cell line SGC- 7901 |
| |
| Institution: | YAO Xuan, ZHANG Ling- min, WANG Yang, YAO Yu ,JI Zong- zheng( 1 Second Affiliated Hospital, Medical School, Xi＇an Jiaotong University, Xi＇an 710004, China ; 2 First Affiliated Hospital , Madical School, Xi＇an Jiaotong Universty ,Xi＇an 710061, China.) |
| |
| Abstract: | Objective：To study the effect of miR -17 -92 cluster on the proliferation and metastasis of human gastric cancer cell line SGC -7901. Methods：After the construction of miR - 17, miR - 18a, and miR -20a antisense oligonucleotides, the effect of them on expression of specific miRNA was confirmed by real - time PCR. The proliferation and metastasis of human gastric cancer cell line SGC - 7901 were detected by M3T assay and transwell assay respectively. Results： Inhibition of miR - 17, miR - 18a, and miR -20a could inhibit the proliferation and invasion of SGC -7901. Conclusion：miR - 17 -92 cluster may acts as a tumor suppresser in human gastric cancer. |
| |
| Keywords: | miR- 17-92 cluster gastric cancer SGC-7901 proliferation metastasis |
| 本文献已被 维普 等数据库收录！ |
|
