EphA2蛋白表达与结直肠癌浸润性及微血管生成的关系

目的：探讨酪氨酸蛋白激酶受体EphA2表达与大肠癌浸润及其与大肠癌微血管生成之间的关系。方法：应用免疫组化方法检测70例大肠癌标本和相应正常大肠黏膜组织中EphA2蛋白的表达情况,分析E-phA2表达与大肠癌临床病理学因素及微血管生成之间的关系。结果：EphA2蛋白在癌组织中的表达明显高于相应正常大肠黏膜组织（P〈0.001）。EphA2蛋白高表达与癌分化程度、生长方式、浸润深度和肿瘤体积相关（P〈0.05）,而与患者的年龄、性别、淋巴结和血行性转移无关（P〉0.05）。免疫组化结果还发现,肿瘤组织内微血管内皮细胞也有EphA2受体蛋白表达。CD34染色后大肠癌的微血管密度（microvessel density,MVD）与EphA2表达水平有显著相关,EphA2阳性表达强度高的肿瘤区域有较高的微血管密度。结论：E-phA2蛋白的高表达与大肠癌的发生和浸润有关。

Correlation of EphA2 expression with invasion and angiogenesis of colorectal carcinoma

Objective：To investigate the expression and significance of erythropoietin-producing hepatocellularA2（EphA2） protein in colorectal carcinoma and the correlation between EphA2 expression and tumor angiogenesis.Methods： Immunohistochemistry was used to examine the EphA2 expression in 70 surgically resected colorectal carcinoma and the paired normal colorectal mucosa tissues.Microvessels in colorectal carcinoma were counted after immunostaining for CD34,and the correlation between EphA2 expression and microvessel counts（MVCs）in colorectal carcinoma were assessed.Results： Expressions of EphA2 protein in carcinoma tissues were obviously higher than that in normal colorectal mucosa tissues（P 0.01）.The overexpression of EphA2 protein correlated with differentiation grades,invasion depth and gross type of carcinomas（P 0.05）,but didn＇t with age,sex of patients,lymph node,and hematogenous metastasis of adenocareinomas（P 0.05）.We observed specific EphA2 staining was present in both cancer cells and tumor vascular endothelial cells.Microvessel density（MVD）after immunostaining for CD34 was significantly correlated with overexpression of EphA2,high microvessel density presented in EphA2-strong positive tumor areas.Conclusion： There is a correlation between EphA2 protein overexpression,and tumorigenesis infiltration of colorectal carcinoma.