首页 | 本学科首页   官方微博 | 高级检索  
     

辐射后淋巴细胞的G显带染色体畸变分析
引用本文:赵明刚,施常备,赵华,袁勇,姚俊涛,陆建荣,袁彬,陈葳,王翔. 辐射后淋巴细胞的G显带染色体畸变分析[J]. 陕西肿瘤医学, 2011, 0(7): 1310-1312
作者姓名:赵明刚  施常备  赵华  袁勇  姚俊涛  陆建荣  袁彬  陈葳  王翔
作者单位:[1]西安交通大学第一附属医院分子医学实验室,陕西西安710061 [2]陕西省肿瘤医院,陕西西安710061
基金项目:陕西省卫生厅科研基金项目(编号2010D10)
摘    要:目的:通过染色体G显带技术研究辐射后染色体的结构畸变情况。方法:4MV的X线辐射离体入血,淋巴细胞培养后,应用染色体G显带技术,分析辐射后染色体的结构畸变情况,包括双着丝粒、缺失、易位、环状染色体、染色体片段,等。结果:辐射后观察分散良好、形态清晰的细胞核型100个,染色体畸变总数为428,其中双着丝粒、缺失、易位、环状染色体、染色体片段的发生数分别为86、41、111、9、181。分析各号染色体辐射后畸变情况,未见明显差异。结论:染色体G带分析可以提供较丰富的染色体结构畸变信息。

关 键 词:辐射  染色体畸变  G显带

G-banding chromosomal aberration assay of lymphocyte after radiation
ZHAO Ming-gang,SHI Chang-bei,ZHAO Hua,YUAN Yong,YAO Jun-tao,LU Jian-rong,YUAN Bin,CHEN Wei,WANG Xiang. G-banding chromosomal aberration assay of lymphocyte after radiation[J]. Shaanxi Oncology Medicine, 2011, 0(7): 1310-1312
Authors:ZHAO Ming-gang  SHI Chang-bei  ZHAO Hua  YUAN Yong  YAO Jun-tao  LU Jian-rong  YUAN Bin  CHEN Wei  WANG Xiang
Affiliation:1First Affiliated Hospital of Medical College of Jiaotong University,Xi'an 710061,China;2Shaanxi Tumor Hospital,Xi'an 710061,China.
Abstract:Objective:To validate the value of G-banding chromosomal aberration of lymphocyte after radiation.Methods: The blood was irradiated by 4 MV X-ray and was cultured 3 days.The G-banding chromosomal aberration was assayed including dicentrics,deletions,translocations,rings,and fragments chromosomal aberration.Results:There were 100 good karyotypes observed.The total of chromosomal aberration was 428.The dicentric,deletion,translocation,ring,and fragment chromosomal aberration were 86,41,111,9,and 181 respectively.There was no significant difference in chromosomal aberration after radiation in every NO.chromosome.Conclusion:The G-banding chromosomal aberration assay should be paid more attention in radiation biology.
Keywords:radiation  chromosomal aberration assay  G-banding technique
本文献已被 维普 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号