可疑翼手参皂苷ColochirosideA体外抑制多种瘤细胞株增殖的研究

目的：研究可疑翼手参体内三萜皂苷ColochirosideA（cA）的体外抗肿瘤活性。方法：用稻瘟霉分生孢子模型对CA进行生物活性初筛，分别用MTT、SRB法检测CA对5种体外培养肿瘤细胞（BEL-7402、HO-8910、MDA—MB-435、MDA—MB-468、A431）和2种体外培养人血管内皮细胞（HMEC、HUVEC）的增殖抑制活性。结果：cA对稻瘟霉显示较强的抑制活性，最小活性浓度（MMDC）〈7．9ug／ml；对5种体外培养人肿瘤细胞显示显著抑制增殖活性，IC50为1．75ug／ml-3．66ug／ml；对2种体外培养人血管内皮细胞均显示显著抑制增殖活性，IC50值分别为2．55ug／ml和5．63ug／ml。结论：CA体外对多种肿瘤细胞具有抑制活性，有进一步研究开发的前景。

Antitumor activities in vitro of the triterpene glycoside Colochiroside A from sea cucumber Colochirus anceps

Objective：To study the antitumor activities in vitro of the triterpene glycoside Colochiroside A （CA） from the sea cucumber Colochirus anceps. Methods： Detecting the deformation of mycelia germinated from.Conidia of Pyricularia oryzae and the antineoplastic activities to 5 types tumor cell （ BEL - 7402, HO - 8910, MDA - MB - 435, MDA -MB -468, A431 ） and 2 types endothelial cell （HMEC, HUVEC） in vitro was tested in demonstrating the effect of the saponin CA. Results ： CA exhibited significant inhibiting activity against Pyricularia oryzae, the preliminary cytotoxic assay of CA exhibited significant cytotoxic activity against 5 types cultured tumor cell lines with the mean of IC50 1.75ug/ml -3.66ug/ml, and the preliminary cytotoxic assay of CA exhibited significant cytotoxic activity against ：2 types cultured tumor cell lines with the IC50 of 5.63 ug/ml, 2.55ug/ml respectively. Conclusion： Saponin CA exhibited remarkable antineoplastic activities in vitro.