可疑翼手参皂苷ColochirosideA体外抑制多种瘤细胞株增殖的研究 |
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引用本文: | 张永娟,易杨华. 可疑翼手参皂苷ColochirosideA体外抑制多种瘤细胞株增殖的研究[J]. 陕西肿瘤医学, 2011, 0(2): 205-207 |
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作者姓名: | 张永娟 易杨华 |
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作者单位: | [1]广东医学院生理学教研室,广东东莞523808 [2]第二军医大学药学院海洋药物研究中心,上海200433 |
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基金项目: | 【基金项目】湛江市科技攻关项目(编号:2009C3101018);广东医学院青年基金项目(编号:XQ06012) |
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摘 要: | 目的:研究可疑翼手参体内三萜皂苷ColochirosideA(cA)的体外抗肿瘤活性。方法:用稻瘟霉分生孢子模型对CA进行生物活性初筛,分别用MTT、SRB法检测CA对5种体外培养肿瘤细胞(BEL-7402、HO-8910、MDA—MB-435、MDA—MB-468、A431)和2种体外培养人血管内皮细胞(HMEC、HUVEC)的增殖抑制活性。结果:cA对稻瘟霉显示较强的抑制活性,最小活性浓度(MMDC)〈7.9ug/ml;对5种体外培养人肿瘤细胞显示显著抑制增殖活性,IC50为1.75ug/ml-3.66ug/ml;对2种体外培养人血管内皮细胞均显示显著抑制增殖活性,IC50值分别为2.55ug/ml和5.63ug/ml。结论:CA体外对多种肿瘤细胞具有抑制活性,有进一步研究开发的前景。
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关 键 词: | 可疑翼手参 三萜皂苷 Colochiroside A 稻瘟霉 MTT法 SRB法 抗肿瘤活性 |
Antitumor activities in vitro of the triterpene glycoside Colochiroside A from sea cucumber Colochirus anceps |
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Affiliation: | ZHANG Yong - juan, YI Yang - hua (1. Department of Physiology, Guangdong Medical College, Dongguan 523808, China ;2 Research Center for Marine Drugs, School of Pharmacy, Second Military Medical University, Shanghai 200433, China. ) |
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Abstract: | Objective:To study the antitumor activities in vitro of the triterpene glycoside Colochiroside A (CA) from the sea cucumber Colochirus anceps. Methods: Detecting the deformation of mycelia germinated from.Conidia of Pyricularia oryzae and the antineoplastic activities to 5 types tumor cell ( BEL - 7402, HO - 8910, MDA - MB - 435, MDA -MB -468, A431 ) and 2 types endothelial cell (HMEC, HUVEC) in vitro was tested in demonstrating the effect of the saponin CA. Results : CA exhibited significant inhibiting activity against Pyricularia oryzae, the preliminary cytotoxic assay of CA exhibited significant cytotoxic activity against 5 types cultured tumor cell lines with the mean of IC50 1.75ug/ml -3.66ug/ml, and the preliminary cytotoxic assay of CA exhibited significant cytotoxic activity against :2 types cultured tumor cell lines with the IC50 of 5.63 ug/ml, 2.55ug/ml respectively. Conclusion: Saponin CA exhibited remarkable antineoplastic activities in vitro. |
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Keywords: | Colochirus anceps triterpene glycoside Colochiroside A Pyricularia oryzae MTT method SRB method antitumor activities |
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