| 三氧化二砷对膀胱癌BIU-87细胞增殖及生存素表达的影响 |
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| 引用本文： | 刘春来,宫雪,刘屹立,王平. 三氧化二砷对膀胱癌BIU-87细胞增殖及生存素表达的影响[J]. 陕西肿瘤医学, 2011, 0(7): 1265-1267 |
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| 作者姓名： | 刘春来 宫雪 刘屹立 王平 |
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| 作者单位： | 中国医科大学附属第四医院泌尿外科,辽宁沈阳110032 |
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| 基金项目： | 辽宁省博士启动基金项目（编号：20101139） |
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| 摘 要： | 目的：探讨三氧化二砷（arsenic trioxide,As2O3）对膀胱癌BIU-87细胞增殖及生存素表达的影响。方法：用As2O3处理膀胱移行细胞癌株BIU-87后,用四甲基偶氮唑蓝法（MTT）检测细胞增殖;用细胞流式仪检测细胞周期及凋亡,生存素（Survivin）表达的变化。结果：三氧化二砷作用BIU-87细胞后,细胞生长受到明显的抑制,Survivin的表达明显减少,凋亡细胞百分比较对照组明显增多,且均具有浓度依赖性。结论：As2O3对人膀胱癌细胞株BIU-87有抑制其生长、增殖和诱导凋亡的作用,且呈现浓度依赖性。
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| 关 键 词： | 膀胱移行细胞癌 BIU-87细胞株 三氧化二砷 凋亡 生存素 |
Expression of Survivin and effect of As_2O_3 on it in bladder cancer cell |
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| LIU Chun-lai,GONG Xue,LIU Yi-li,WANG Ping. Expression of Survivin and effect of As_2O_3 on it in bladder cancer cell[J]. Shaanxi Oncology Medicine, 2011, 0(7): 1265-1267 |
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| Authors: | LIU Chun-lai GONG Xue LIU Yi-li WANG Ping |
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| Affiliation: | Department of Urology,The Fourth Affiliated Hospital of China Medical University,Shenyang 110032,China. |
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| Abstract: | Objective：To investigate the effect of As2O3 on Survivin in BIU-87 cell line.Methods：To treat the BIU-87 cells by As2O3 at different concentration,then detect the proliferation activity of BIU-87 by MTT assay,while the expression of Survivin,cell cycle and apoptosis by flow cytometry.Results：After treated with As2O3,the proliferation activity of BIU-87 cells were significantly slowed down compare to the control group,with an evident time and dose-response relationship.As2O3 can induce the apoptosis of BIU-87 cells with concentration dependent.After cultured with As2O3 the expression of Survivin was downregulated significantly,which were concentration dependent.Conclusion：As2O3 can inhibit proliferation activity of BIU-87 cell and reduce the expression of Survivin,induce the apoptosis of BIU-87 cell with concentration dependant. |
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| Keywords: | bladder transitional cell carcinoma BIU-87 cell line arsenic trioxide apoptosis Survivin |
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