| KOZAK序列对乳腺癌MDA-MB-231细胞CCNL1基因表达的影响 |
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| 引用本文： | 李鹏,马艳娇,徐晓芳,宫鹏涛,杨举,李建华,李赫,欧阳红生,张西臣. KOZAK序列对乳腺癌MDA-MB-231细胞CCNL1基因表达的影响[J]. 陕西肿瘤医学, 2011, 0(7): 1259-1262 |
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| 作者姓名： | 李鹏 马艳娇 徐晓芳 宫鹏涛 杨举 李建华 李赫 欧阳红生 张西臣 |
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| 作者单位： | [1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319 [2]吉林大学畜牧兽医学院,吉林长春130062 |
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| 基金项目： | 国家重大基础研究计划资助项目（编号：2006CB-910505） |
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| 摘 要： | 目的：探讨KOZAK序列对细胞周期调节蛋白1（CCNL1）基因在人乳腺癌细胞MDA-MB-231中表达的影响。方法：构建pcDNA3.1-CCNL1和pcDNA3.1-CCNL1-K（含有KOZAK序列）的真核重组表达质粒,采用Fugene HD转染试剂将重组表达质粒转染入MDA-MB-231细胞,并用荧光定量PCR和Western blot方法检测pcDNA3.1-CCNL1和pcDNA3.1-CCNL1-K重组质粒在MDA-MB-231细胞中表达的差异。结果：在MDA-MB-231细胞中质粒pcDNA3.1-CCNL1-K比空白对照细胞mRNA和蛋白质表达量要高;而质粒pcDNA3.1-CCNL1比空白对照细胞mRNA和蛋白质表达量也高。结论：在MDA-MB-231细胞中,质粒pcDNA3.1-CCNL1-K较pcDNA3.1-CCNL1的mRNA和蛋白表达水平均存在差异,KOZAK序列能够提高CCNL1基因在MDA-MB-231细胞中的表达。
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| 关 键 词： | KOZAK序列 细胞周期调节蛋白1 乳腺癌 MDA-MB-231细胞 |
Enhancement of cyclin L1 expression by a key sequence-KOZAK in MDA-MB-231 cells |
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| LI Peng,MA Yan-jiao,XU Xiao-fang,GONG Peng-tao,YANG Ju,LI Jian-hua,LI He,OUYANG Hong-sheng,ZHANG Xi-chen. Enhancement of cyclin L1 expression by a key sequence-KOZAK in MDA-MB-231 cells[J]. Shaanxi Oncology Medicine, 2011, 0(7): 1259-1262 |
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| Authors: | LI Peng MA Yan-jiao XU Xiao-fang GONG Peng-tao YANG Ju LI Jian-hua LI He OUYANG Hong-sheng ZHANG Xi-chen |
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| Affiliation: | 1Institute of Animal Science and Technology,Heilongjiang Bayi Agriculture University,Daqing Heilongjiang 163319,China;2College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China. |
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| Abstract: | Objective： To explore whether the expression of CCNL1 gene and the KOZAK sequence can enhance the expression of CCNL1 gene in breast cancer cells（MDA-MB-231）.Methods：The eukaryotic expression plasmids（pcDNA3.1-CCNL1 and pcDNA3.1-CCNL1-K） were constructed.They were transfected into the MDA-MB-231 cells by Fugene HD transfection reagents.Detect the expression of pcDNA3.1-CCNL1 and pcDNA3.1-CCNL1-K in the MDA-MB-231 cells by real time PCR and Western blot.Results：The KOZAK sequence could enhance the expression of mRNA and protein in the MDA-MB-231 cells.Conclusion：KOZAK sequence play a key role in CCNL1 protein expression. |
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| Keywords: | KOZAK sequence CCNL1 breast cancer MDA-MB-231 cell |
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