| 尿路肿瘤细胞多重荧光PCR检测微卫星不稳定 |
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| 引用本文： | 吴晓斌,赖仁胜,郑燕影,孙怡.尿路肿瘤细胞多重荧光PCR检测微卫星不稳定[J].陕西肿瘤医学,2011(8):1614-1616. |
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| 作者姓名： | 吴晓斌 赖仁胜 郑燕影 孙怡 |
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| 作者单位： | 南京中医药大学附属医院病理科/国家中管局三级分子生物实验室,江苏南京210029 |
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| 摘 要： | 目的：研究膀胱肿瘤尿路脱落细胞的微卫星状态及其临床病理关系。方法：35例膀胱肿瘤尿液脱落细胞应用多重荧光PCR检测尿路的微卫星状态。结果：35例尿液样本：膀胱肿瘤组15例,有6例MSI-H,8例MSI-L,1例MSS,诊断阳性敏感度达93.33%;膀胱癌术后复查组6例,血尿及尿路上皮轻度异型组7例,正常体检组7例中,在血尿及轻度异型组发现1例MSI-L,其余均为MSS,阴性特异率达95%。结论：尿路脱落细胞的微卫星检测可作为膀胱肿瘤诊断和监测复发的有效非侵袭性检测方法。
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| 关 键 词： | 尿路脱落细胞 微卫星不稳定 多重荧光PCR |
Urinary tumor cells of multiple fluorescent PCR detection for microsatellite instability |
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| WU Xiaobin,LAI Rensheng,ZHENG Yanying,SUN Yi.Urinary tumor cells of multiple fluorescent PCR detection for microsatellite instability[J].Shaanxi Oncology Medicine,2011(8):1614-1616. |
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| Authors: | WU Xiaobin LAI Rensheng ZHENG Yanying SUN Yi |
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| Institution: | Department of Pathology,the Affiliated Hospital of Nanjing University of Traditional Chinese Medicine,the Third-Grade Laboratory of Molecular Biology,State Administration of Traditional Chinese Medicine,Jiangsu Nanjing 210029,China |
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| Abstract: | Objective：To study urinary cytology of bladder tumor mirosatellite instablity and the relationship with clinicopathology.Methods： Multiplex PCR fluorescent detection of urinary exfoliated the cells status of mirosatellite instablity.Results： There were 6 high microsatellite instability,8 low microsatellite instability,and 1 normal microsatellite stability among 15 urinary cancer patients;only one low microsatellite instability was founded among 6 post-operated bladder cancer patients,7 urinary system inflammation and dysplasia patients,and 7 medical examination normal persons.Conclusion： Microsatellite instablity urinary cytology may be used as diagnosis and monitoring of bladder cancer recurrence and effective non-invasive detection. |
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| Keywords: | urinary cytology mirosatellite instablity multiple fluorescent PCR |
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