紫杉醇提高胃癌MGC803细胞对TRAIL的敏感性

目的：研究紫杉醇对TRAIL诱导胃癌细胞凋亡的影响,探讨死亡受体5（DR5）在TRAIL诱导细胞凋亡中的作用。方法：采用MTT法测定细胞活力,流式细胞仪检测细胞凋亡,western blot检测蛋白表达。结果：在MGC803细胞中,100ng/ml的TRAIL导致轻度的增殖抑制和细胞凋亡,TRAIL（100ng/ml）联合紫杉醇（3.41g/ml）引起明显的增殖抑制和细胞凋亡（P〈0.05）。TRAIL单药没有改变死亡受体5（DR5）的表达,而3.41g/ml紫杉醇作用MGC803细胞后明显上调了DR5的表达。结论：紫杉醇通过上调DR5的表达增强TRAIL诱导的胃癌MGC803细胞凋亡。

Paclitaxel increases the senisitivity of gastric cancer MGC803 cells to TRAIL

Objective：To assess the effect of paclitaxel on TRAIL-induced apoptosis in gastric cancer cells,and the action of death receptor 5（DR5） in TRAIL-induced apoptosis.Methods：Cell proliferation was measured using MTT assay.Cell apoptosis was determined by flow cytometry.Expression of proteins was analyzed by western blot.Results：Treatment with 100 ng/ml TRAIL for 24 h resulted in a slight reduction in cell viability and a little increase in cell apoptosis in MGC803 cells.Treatment with TRAIL（100 ng/ml） and paclitaxel（3.41 g/ml） leaded to a significant reduction in cell viability and a dramatic increase in cell apoptosis（P0.05）.TRAIL alone did not change the expression of death receptor 5（DR5）,while 3.41 g/ml paclitaxel significantly upregulated the expression of DR5 in MGC803 cells.Conclusion：Paclitaxel enhanced TRAIL-induced apoptosis in gastric cancer MGC803 cells by upregulating the expression of DR5.