发光二极管光动力作用对人肺腺癌细胞株A549的增殖抑制效应

目的：探讨以发光二极管（Light-emitting diode,LED）作为光动力学疗法（photodynamic therapy,PDT）的光源,对人肺腺癌细胞株A549体外增殖的影响及其与传统大功率半导体激光器的比较。方法：采用肺腺癌A549细胞常规培养并传代,将细胞接种于底部澄清黑色96孔板内培养,加入相同浓度的血卟啉衍生物类光敏剂photosan3,分别用LED激光器（光功率为50mW）和大功率半导体激光器（光功率为200mW）采用相同光能量密度（分为三组：5J/cm^2、10 J/cm^2、20J/cm^2）进行照射。照光后用MTT法检测不同激光器PDT作用后细胞OD492值,并计算细胞存活率。结果：在光敏剂浓度相同、能量密度为5、10、20J/cm^2的条件下进行PDT,结果表明与大功率半导体激光器相比,以LED为光源对肺腺癌A549细胞进行PDT后细胞存活率更低,各组相互比较有统计学差异（P〈0.05）。结论：在相同光敏剂浓度及能量密度下采用低功率、长时间照射的LED-PDT组较半导体激光器PDT组,细胞存活率更低,LED-PDT对肺腺癌细胞株A549具有更好的杀伤效果。

Effect of LED-PDT on human lung adenocarcinoma cell line A549

Objective：To observe the effect of light-emitting diodes（LED） as an alternative light source in photodynamic therapy（PDT） on human lung adenocarcinoma cell line A549 in vitro.To compare the different effectiveness of LED and high-power semiconductor laser on human lung adenocarcinoma cell line A549 in vitro.Methods： The lung adenocarcinoma A549 cells were resustated,conventional cultured and subcultured.The cultured lung adenocarcinoma A549 cells were inoculated at the clear bottom black 96-well plate and exposed at intensities of 5J/cm^2,10 J/cm^2,20J/cm^2 in LED laser（power 50mW）and high-power semiconductor laser（power 200mW）respectively.MTT assay was applied to measure the OD492 of each group with different light source,and then the rate of cell remnants was calculated.Results： The average cell survival rate of LED-PDT group was lower than the result of high-power semiconductor laser.Compared with semiconductor laser,the cell survival rate of LED-PDT group was lower.When under the same photosensitizer concentration,the cell survival rate was significantly different in each group（P〈0.05）.Conclusion： Compared the effect of LED and semiconductor laser on lung cancer A549 cell line,the effect of LED-PDT is more powerful at the same concentration of photosensiser and laser intensity.