A simple,robust and rapid approach to detect carbapenemases in Gram-negative isolates by MALDI-TOF mass spectrometry: validation with triple quadripole tandem mass spectrometry,microarray and PCR |
| |
Institution: | 1. Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland;2. Laboratory of Clinical Pharmacology Innovation and Development Unit, Service of Biomedicine, Lausanne, Switzerland |
| |
Abstract: | Carbapenemases should be accurately and rapidly detected, given their possible epidemiological spread and their impact on treatment options. Here, we developed a simple, easy and rapid matrix-assisted laser desorption ionization-time of flight (MALDI-TOF)-based assay to detect carbapenemases and compared this innovative test with four other diagnostic approaches on 47 clinical isolates. Tandem mass spectrometry (MS-MS) was also used to determine accurately the amount of antibiotic present in the supernatant after 1 h of incubation and both MALDI-TOF and MS-MS approaches exhibited a 100% sensitivity and a 100% specificity. By comparison, molecular genetic techniques (Check-MDR Carba PCR and Check-MDR CT103 microarray) showed a 90.5% sensitivity and a 100% specificity, as two strains of Aeromonas were not detected because their chromosomal carbapenemase is not targeted by probes used in both kits. Altogether, this innovative MALDI-TOF-based approach that uses a stable 10-μg disk of ertapenem was highly efficient in detecting carbapenemase, with a sensitivity higher than that of PCR and microarray. |
| |
Keywords: | Carbapenem resistance Gram-negative bacilli MALDI-TOF MS microarray MS-MS spectrometry PCR |
本文献已被 ScienceDirect 等数据库收录! |
|