| 尿蛋白电泳在肾脏疾病诊断中的临床应用 |
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| 引用本文： | 李贵芳,王树辉,夏凤琼,陈铭希.尿蛋白电泳在肾脏疾病诊断中的临床应用[J].检验医学与临床,2011,8(19):2353-2354. |
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| 作者姓名： | 李贵芳 王树辉 夏凤琼 陈铭希 |
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| 作者单位： | 1. 贵州省人民医院检验科,贵阳,550002
2. 贵阳医学院检验系实习生,550002 |
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| 摘 要： | 目的根据蛋白质分子量不同,采用十二烷基硫酸钠琼脂糖凝胶(SDS-AGE)对尿蛋白进行电泳,以判断肾脏疾病的受损部位及病变程度。方法采用法国Sebia公司HYDRASYS全自动电泳分析仪对104例肾脏疾病患者尿液进行电泳分析。结果根据尿蛋白电泳图谱扫描测定,104例尿蛋白电泳显示10例为生理性蛋白尿,37例为肾小球性蛋白尿,12例为肾小管性蛋白尿,45例为混合性蛋白尿,其中20例同时做肾活检,系膜增生性肾小球肾炎18例,膜增生性肾小球肾炎2例,SDS-AGE尿蛋白电泳结果与肾活检结果基本相符。结论琼脂糖尿蛋白电泳技术简便快速,无创伤,对早期肾损伤的诊断,肾脏损伤部位分析,指导治疗和判断预后有较高的价值。
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| 关 键 词： | 尿蛋白电泳 琼脂糖凝胶 肾脏疾病 |
Clinical application of urine protein electrophoresis in diagnosis of kidney diseases |
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| LI Gui-fang,WANG Shu-hui,XIA Feng-qiong,CHEN Ming-xi.Clinical application of urine protein electrophoresis in diagnosis of kidney diseases[J].Laboratory Medicine and Clinic,2011,8(19):2353-2354. |
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| Authors: | LI Gui-fang WANG Shu-hui XIA Feng-qiong CHEN Ming-xi |
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| Institution: | 1.Department of Clinical Laboratory,People′s Hospital of Guizhou,Guiyang 550002,China;2.Department of Medical Laboratory,Guiyang Medical College 550002,China) |
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| Abstract: | Objective To explore the position and severity of kidney diseases according to the urine protein molecular weight by urine protein electrophoresis with sodium dodecyl sulfate-agarose gel(SDS-AGE).Methods Urine samples obtained from 104 patients with kidney diseases were examined by urine protein electrophoresis on a HYDRASYS automated gel electrophoresis apparatus(Sebia).Results According to the results of electrophoresis,104 urine samples were classified as the following categories:10 were physiological pr... |
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| Keywords: | urine protein electrophoresis SDS-AGE kidney disease |
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