| 梅毒检测方法的比较分析 |
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| 引用本文： | 阮光强,陈卫. 梅毒检测方法的比较分析[J]. 检验医学与临床, 2011, 8(20): 2470-2471. DOI: 10.3969/j.issn.1672-9455.2011.20.019 |
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| 作者姓名： | 阮光强 陈卫 |
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| 作者单位： | 1. 广东省台山市中心医院检验科,529200
2. 四川新成生物科技有限责任公司,成都,611731 |
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| 摘 要： | 目的了解广东省台山市梅毒感染情况,对梅毒检测方法学进行评价,为制订相应的干预和实验室检测策略提供依据。方法采用快速血浆反应素试验(RPR)和梅毒螺旋体酶联免疫吸附试验(TP-ELISA)方法进行检测,阳性结果均用梅毒螺旋体明胶凝集试验(TPPA)进行确认。结果 2010年共596份血清样本(上半年319份,下半年277份),在TPPA法确诊的45份阳性标本中,RPR法检出阳性16份,敏感性为35.56%;ELISA法检出阳性43份,敏感性为95.56%。RPR法共检出阳性21份,有5份确诊为阴性,特异性为99.16%;ELISA法共检出阳性46份,有3例确诊为阴性,特异性为99.50%。RPR和TPPA阳性结果差异有统计学意义(P<0.05),ELISA与TPPA阳性结果差异无统计学意义(P>0.05)。结论 ELISA检测法比RPR检测法的敏感性高,特异性好,可作为梅毒感染的一种理想检测方法,对控制梅毒的性传播有重要意义。但对ELISA阳性标本应做TPPA确认。
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| 关 键 词： | 梅毒 快速血浆反应素试验 梅毒螺旋体酶联免疫吸附试验 梅毒螺旋体明胶凝集试验 |
Comparative analysis of detection methods of syphilis |
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| RUAN Guang-qiang,CHEN Wei. Comparative analysis of detection methods of syphilis[J]. Laboratory Medicine and Clinic, 2011, 8(20): 2470-2471. DOI: 10.3969/j.issn.1672-9455.2011.20.019 |
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| Authors: | RUAN Guang-qiang CHEN Wei |
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| Affiliation: | RUAN Guang-qiang1,CHEN Wei2(1.Department of Clinical Laboratory,Central Hospital of Taishan City,Guangdong 529200,China,2.Sichuan Sinew Bio-technology Co.,LTD.,Chengdu,Sichuan 611731,China) |
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| Abstract: | Objective To assess the syphilis infection situation,and to evaluate syphilis testing methods and provide an appropriate intervention and laboratory testing strategy.Methods We took rapid plasma reagin(RPR) and enzyme-linked immunosorbent assay(ELISA) as the testing methods and positive results were confirmed with treponema pallidum agglutination test(TPPA).Results There were 596 serum samples this year(319 in the first half year and 277 in the rest).Among the 45 TPPA positive samples,16 samples turned out ... |
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| Keywords: | syphilis rapid plasma reagin treponema pallidum-enzyme-linked immunosorbent assay treponema pallidum agglutination test |
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