G实验和真菌培养联合检测对临床深部真菌感染的诊断价值

目的评估血清1,3-β-D-葡聚糖定量分析(G实验)和真菌培养联合检测对临床深部真菌感染的诊断意义。方法通过回顾性分析75例怀疑深部真菌感染住院患者的G实验和真菌培养结果,分别计算两种方法及联合检测的灵敏度、特异度、阳性和阴性预测值。结果 G实验和真菌培养联合检测的灵敏度、特异度、阳性和阴性预测值分别为88.0%、96.0%、91.7%和94.1%。G实验的灵敏度和阳性预测值高,真菌培养的特异度高。结论G实验和真菌培养联合检测可提高实验的灵敏度,减少假阴性的发生,对早期诊断深部真菌感染有一定临床意义。

Value of G-test combined with fungal culture on diagnosis of deep fungal infections

Objective To investigate the clinical significance of 1,3-β-D-glucan（G-test） combined with fungal culture on the diagnosis of deep fungal infections（DFI））. Methods To retrospectively analyze the result of G-test and fungal culture in 75 inpatients with suspected deep fungal infections. The sensitivity, specificity, positive and negative predictive values for the two methods and combination detection were calculated respectively. Results The sensitivity and specificity of both G-test and fungal culture were 88.0% and 96.0 %, and the positive and negative predictive values were 91.7% and 94. 1% respectively. The performance of G-test diagnosis appeared to be better for sensitivity. Fungal culture was shown to haves a higher specificity than G-test. Conclusion G-test combined with fungal culture increases the sensitivity to the diagnosis of deep fungal infections and decreases false negative cases, which could be a more reliable method for early diagnosis of DFI.