血管内皮生长因子和基质金属蛋白酶-9在非小细胞肺癌中的表达及临床意义

目的研究非小细胞肺癌（NSCLC）组织中血管内皮生长因子（VEGF）及基质金属蛋白酶-9（MMP-9）的表达情况,探讨VEGF及MMP-9在NSCLC侵袭和转移中的作用。方法应用免疫组织化学SABC法检测23例肺良性病变组织、30例NSCLC癌旁正常肺组织及111例NSCLC组织中VEGF及MMP-9的表达水平。结果NSCLC组织中VEGF及MMP-9的阳性表达率均高于肺良性病变组织和NSCLC癌旁正常肺组织（P〈0.05）。VEGF和MMP-9的表达随组织分化程度的降低而升高（P〈0.05）;在腺癌中MMP-9的阳性表达率高于鳞癌（P〈0.05）。VEGF和MMP-9的阳性表达率随TNM分期增高而升高（P〈0.05）,VEGF的阳性表达率随肿瘤增大而升高（P〈0.05）。有淋巴结转移者VEGF和MMP-9的阳性表达率高于无淋巴结转移者（P〈0.05）;存活1 a以内、1-3 a的患者VEGF和MMP-9的阳性表达率均高于存活3-5 a者（P〈0.05）。结论VEGF和MMP-9可能在NSCLC的侵袭和转移中发挥重要作用,可作为评估NSCLC的侵袭、转移和预后的参考指标。

The Expression and Significance of VEGF and MMP-9 in Non-small Cell Lung Cancer

Objective To determine the expression of Vascular Endothelial Growth Factor（VEGF） and Matrix Metalloproteinases-9（MMP-9） in non-small cell lung cancer（NSCLC） specimens and try to find out the possible roles of VEGF and MMP-9 in the invasion and metastasis of NSCLC.Methods Immunohistochemical SABC method was used to detect the expression of VEGF and MMP-9 in 23 non-cancerous pulmonary diseases tissues,30 normal lung tissues near the NSCLC and 111 NSCLC tissues.Results The expression levels of VEGF and MMP-9 in the NSCLC tissues were significantly higher than those of the control groups（P〈0.05）.The expression of VEGF and MMP-9 were higher in low differentiated lung cancer tissue than those in higher differentiated lung cancer tissues（P〈0.05）.The positive rate of MMP-9 in adenocarcinoma was remarkably higher than that in squamous cell carcinoma（P〈0.05）.Expression rate of VEGF and MMP-9 had positive relation with P-TNM stage（P〈0.05）.Expression rate of VEGF had positive relation with tumor volume（P〈0.05）.The positive rate of VEGF and MMP-9 were significantly higher in tumors with lymph node metastasis than those without lymphatic metastasis（P〈0.05）.The patients survival for 1 and 3 years had much higher VEGF and MMP-9 expression rate than those for 5 years（P〈0.05）.Conclusion VEGF and MMP-9 may play important roles in NSCLC invasion and metastasis,their expression may act as a reference to evaluate metastasis and prognosis of NSCLC.