邻苯酸二甲酯（塑化剂）对大鼠肾脏及生殖系统的影响

目的探索邻苯酸二甲酯（塑化剂）对大鼠肾脏及生殖系统的影响。 方法Wistar清洁级雄性大鼠60只,随机数字表法分为低剂量组、中剂量组、高剂量组和对照组,每组15只,用药组分别给予剂量100、500、1 000 mg/（kg·d）的邻苯酸二甲酯,对照组仅给予植物油。6周后检测血清肌酐（Cr）、尿素氮（BUN）、黄体生成素（LH）、卵泡刺激素（FSH）、睾酮（T）、胱抑素C（Cys-C）,β2-微球蛋白（β-Mg）水平。HE染色观察肾脏组织形态学变化。组间比较采用单因素方差分析。 结果高剂量组Cr、BUN明显升高（F=11.70,4.16;P<0.05）,而Cys-C、β2-微球蛋白在低、中、高剂量组均明显升高（F=31.16,8.05;P<0.05）。高剂量组中血清睾酮水平降低;低剂量组LH水平升高,而中剂量组、高剂量组血清LH水平降低;低、中、高剂量组血清FSH水平升高（F=4.82,59.34,6.91;P<0.05）。对照组和低剂量组大鼠肾脏组织结构未见明显改变;中剂量组大鼠肾脏中肾小球出现萎缩,高剂量组大鼠肾小球出现萎缩,变性,坏死。 结论邻苯酸二甲酯可引起大鼠生殖内分泌激素水平的变化和肾小球病变。

Effect of dimethyl phthalate on kidney and reproductive system in rats

ObjectiveTo study the effect of dimethyl phthalate on kidney and reproductive system in rats. MethodsSixty Wistar clean grade male rats were randomly divided into low dose group, middle dose group, high dose group and control group with 15 rats in each group. Serum creatinine (Cr), urea nitrogen bun (BUN), luteinizing hormone (LH), follicle stimulating hormone (FSH), testosterone (T), cystatin C (cys-C), β-2 microglobulin (β-Mg) were detected in the drug groups after 6 weeks of treatment.The changes of renal histomorphology were observed by HE staining. Single factor analysis of variance was used to compare the two groups. ResultsSerum creatinine and BUN increased significantly in high dose group (F=11.70, 4.16; P<0.05), while Cystatin C (Cys-C) and β-2-microglobulin (β-Mg) increased significantly in low, medium and high dose groups. (F=31.16, 8.05; P<0.05). Serum testosterone level decreased in high dose group, LH level increased in low dose group, but decreased in high dose group and middle dose group, FSH level increased in low, medium and high dose group (F=4.82, 59.34, 6.91; P<0.05). There was no obvious change in renal tissue structure in the control group and low dose group, while the glomerular atrophy occurred in the kidney in the middle dose group, and the glomerular atrophy, degeneration and necrosis occurred in the high dose group. ConclusionDimethyl phthalate can induce the changes of reproductive endocrine hormone level and glomerular lesion in rats.