十株产色素非O1群霍乱弧菌的发现与研究

目的研究产色素非Ｏ１群霍乱弧菌在水源水中的分布、生物学性状及其致病性。方法在本地主要饮用水源设点、采样、培养致病性弧菌。分离、鉴定该菌过程中用营养琼脂斜面代替双糖铁琼脂取得良好效果。结果１９９４～１９９７年在水源水中相继检出１０株产色素的革兰阴性弧状菌，经形态学、培养特性、生化学、血清学的系统鉴定，均符合非Ｏ１群霍乱弧菌的定义，动物实验等显示其有较强毒力。结论因该菌在碱性胆盐琼脂、营养琼脂斜面和血琼脂上培育后能产生水溶性褐色色素，故命名为产色素非Ｏ１群霍乱弧菌

Discovery of Ten Strains of Non O1 Vibrio Cholerae Pigmentogens

Objective To study the distribution of non O1 Vibrio cholerae pigmentogens in water and its biological characteristics and pathogenicity. Methods Samples were collected from local source of drinking water and cultured for pathogenic V. cholerae. Non O1 V. cholerae was isolated and identified with nutrient agar slant instead of KIA. Results Ten strains of V. cholerae producing pigment with negative Gram's stain were detected in the source water in Lu'an Prefecture, Anhui Province during 1994 to 1997, which could all met the definition of non O1 V. cholerae in morphology, cultural characteristics, biochemistry and serology. with systematic identification. Animal experiments showed that it had strong virulence. Conclusion These strains of V. cholerae cultured in basic cholate agar, nutrient agar slant and blood agar could produce brown pigment, so it was called non O1 V. cholerae pigmentogens.