| HPLC测定冬凌草中迷迭香酸、冬凌草甲素和猫眼草黄素的含量 |
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| 引用本文： | 袁兴利,闫利华,张启伟,王智民. HPLC测定冬凌草中迷迭香酸、冬凌草甲素和猫眼草黄素的含量[J]. 中国中药杂志, 2013, 38(14): 2343-2347 |
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| 作者姓名： | 袁兴利 闫利华 张启伟 王智民 |
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| 作者单位： | 中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700;天津中医药大学, 天津 300193;中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700;中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700;中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700 |
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| 基金项目： | 国家"重大新药创制"科技重大专项(2009ZX09308-003, 2009ZX09301-005);中国中医科学院自主选题项目(2011xycz-11) |
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| 摘 要： | 建立同时测定冬凌草药材中迷迭香酸、冬凌草甲素和猫眼草黄素含量的高效液相色谱方法。采用Ultimate C18色谱柱(4.6 mm×250 mm, 5 μm);流动相甲醇-0.1%甲酸水溶液,梯度洗脱;流速1.0 mL·min-1;柱温30℃;检测波长338,242 nm。迷迭香酸、冬凌草甲素和猫眼草黄素的线性范围分别为0.222~2.78,0.227~2.84,0.005 68~0.071 μg;平均加样回收率分别为102.9%(RSD 1.9%),99.6%(RSD 1.1%),102.5%(RSD 0.94%) 。该方法简便、准确,重复性好,可用于冬凌草药材的质量控制。
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| 关 键 词： | 冬凌草 迷迭香酸 冬凌草甲素 猫眼草黄素 含量测定 高效液相色谱法 |
| 收稿时间： | 2013-03-26 |
Simultaneous determination of rosmarinic acid, oridonin and chrysoplenetin in Isodon rubescens by HPLC |
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| YUAN Xing-li,YAN Li-hu,ZHANG Qi-wei and WANG Zhi-min. Simultaneous determination of rosmarinic acid, oridonin and chrysoplenetin in Isodon rubescens by HPLC[J]. China Journal of Chinese Materia Medica, 2013, 38(14): 2343-2347 |
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| Authors: | YUAN Xing-li YAN Li-hu ZHANG Qi-wei WANG Zhi-min |
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| Affiliation: | Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China |
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| Abstract: | |
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| Keywords: | Isodon rubescens rosmarinic acid oridonin chrysoplenetin assay HPLC |
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