氟烷吸入麻醉引起c—fos基因在中枢神经系统的表达与分布

研究氟烷吸入麻醉在中枢神经系统作用部位。应用ｃ－ｆｏｓ基因免疫组织化学方法。ＳＤ大鼠在吸入氟烷浓度为０．７５％．１。５％和２％麻醉１ｈ后，Ｆｏｓ阳性神经元主要分布于端脑；梨状皮层，杏仁核簇，伏核，外侧隔核，终纹麻核、海马ＣＡＩ区，海马回嗅觉小岛；

Expression and distribution of c-fos oncogene within central nervous system of the rat following halothane anesthesia

Objective: The distribution of c-fos oncogene expression within central nervous system (CNS) of the rat was studied during halothane anesthesia. Methods: c-fos oncogene immunohistochemical technique (ABC method) was employed. Results: When halothane concentration was 0. 75%, 1. 5% and 2%, most of the fos-like immunoreactive neurons (FLN) appeared in telencephalon, diencephalon and brain stem which included piriform cortex, amydaloid nucleus cluster, accumbens nucleus, lateral septal nucleus, bed nucleus of the stria terminalis, field CA1 of Ammon's horn, islands of calleja, paraventricular thalamic nucleus, central medial thalamic nucleus, reuniens thalamic nucleus, rhomboid thalamic nucleus, ventral posterolateral thalamic nucleus, paraventricular hypothalamic nucleus (ventral part), periventricular hypothalamic nucleus, median preoptic nucleus, supraoptic nucleus, suprachiasmatic nucleus, medidal and lateral habenular nucleus , midbrain periaqueductal gray and edinger westphal nucleus. In the experiment, it is also discovered that the mumber of FLN were adding steadily along with the increase of the concentration of halothamic. Conclusion: It is obvious that the FLN plays an important role in process of halothane anesthesia and lay the foundation for us to further study the mechanism of the general anesthesia.