| 2-甲氧基雌二醇大鼠血浆蛋白结合率的测定 |
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| 引用本文： | 李杨,张青青,孙惠斌,马思佳,金建闻,张振中,杜斌. 2-甲氧基雌二醇大鼠血浆蛋白结合率的测定[J]. 中国新药与临床杂志, 2011, 0(10) |
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| 作者姓名： | 李杨 张青青 孙惠斌 马思佳 金建闻 张振中 杜斌 |
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| 作者单位： | 河南省食品药品检验所;郑州大学药学院; |
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| 摘 要： | 目的建立测定2-甲氧基雌二醇大鼠血浆蛋白结合率的方法。方法采用平衡透析法对2-甲氧基雌二醇大鼠血浆蛋白结合率进行HPLC测定。结果高、中、低3个浓度(2、4、8 mg.L-1)的2-甲氧基雌二醇血浆蛋白结合率分别为(85.2±3.4)%、(82.1±5.3)%、(83.0±5.2)%。结论该方法灵敏度高,重现性好,操作简单,能满足生物样品分析要求。2-甲氧基雌二醇大鼠血浆蛋白结合率不具有浓度依赖性,使其临床用药具备较好的安全性。
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| 关 键 词： | 2-甲氧基雌二醇 血浆 蛋白结合率 平衡透析 色谱法,高压液相 |
Determination of rat plasma protein binding rate of 2-methoxyestradiol |
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| LI Yang,ZHANG Qing-qing,SUN Hui-bin,MA Si-jia,JIN Jian-wen,ZHANG Zhen-zhong,DU Bin. Determination of rat plasma protein binding rate of 2-methoxyestradiol[J]. Chinese Journal of New Drugs and Clinical Remedies, 2011, 0(10) |
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| Authors: | LI Yang ZHANG Qing-qing SUN Hui-bin MA Si-jia JIN Jian-wen ZHANG Zhen-zhong DU Bin |
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| Affiliation: | LI Yang1,ZHANG Qing-qing2,SUN Hui-bin2,MA Si-jia2,JIN Jian-wen2,ZHANG Zhen-zhong2,DU Bin2(1.He-nan Provincial Institute of Food and Drug Control,Zhengzhou HE-NAN 450003,China,2.School of Pharmacy,Zhengzhou University,Zhengzhou HE-NAN 450001,China) |
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| Abstract: | AIM To develop a HPLC method for the determination of the plasma protein binding rate of 2-methoxyestradiol(2-ME)in rat.METHODS The equilibrium dialysis was carried out to determine the protein binding rate of 2-ME in rat plasma.RESULTS The plasma protein binding rates of 2-ME at high,middle and low concentrations(2,4,8 mg·L-1)were(85.2 ± 3.4)%,(82.1 ± 5.3)%,(83.0 ± 5.2)%,respectively.CONCLUSION The method is sensitive,accurate,simple and reproducible,and meets the requirement of biological sample analysis.The plasma protein binding rate of 2-ME shows no concentration dependence,which provides better security for clinical medication. |
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| Keywords: | 2-methoxyestradiol plasma protein binding rate equilibrium dialysis chromatography high pressure liquid |
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