福建棘隙吸虫与相关虫种随机引物扩增多态DNA分析及感染实验观察

目的：探讨福建棘隙吸虫与相关虫种分类问题。方法：应用 ２８ 个随机引物扩增多态性 Ｄ Ｎ Ａ，根据福建棘隙吸虫、藐小棘隙吸虫（安徽）与日本棘隙吸虫（福建和江西）作相似性分析；现场采检叶形棘隙吸虫（湖北）、藐小棘隙吸虫（安徽）和福建棘隙吸虫囊蚴进行人工感染实验及形态观察。结果：分析福建、安徽与江西省 ３ 种棘隙吸虫的 ４６９ 个多态 Ｄ Ｎ Ａ 片段，福建棘隙吸虫与日本棘隙吸虫福建株、日本棘隙吸虫江西株与福建株、福建棘隙吸虫与安徽的藐小棘隙吸虫间基因组 Ｄ Ｎ Ａ 的 Ｒ Ａ Ｐ Ｄ 相似率分别为 ２０．８％ 、９９．７％ 及 ９７．６％ 。湖北、安徽和福建 ３ 省均分别获得叶形棘隙吸虫、福建棘隙吸虫及日本棘隙吸虫 ３ 种棘隙吸虫。结论：福建棘隙吸虫与日本棘隙吸虫为两个独立虫种，安徽的藐小棘隙吸虫与福建棘隙吸虫为同一虫种。湖北、安徽与福建 ３ 省均存在叶形棘隙吸虫、福建棘隙吸虫和日本棘隙吸虫 ３ 种吸虫的混合感染，其中福建棘隙吸虫较为常见

TAXONOMIC STUDIES ON ECHINOCHASMUS FUJIENENSIS AND ITS RELATED SPECIES BY RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS AND EXPERIMENTAL INFECTION

AIM:To explore the identification and differentiation of Echinochasmus fujianensis,Echinochasmus japonicus(Jiangxi strain and Fujian strain), Echinochasmus liliputanus Anhui and Echinochasmus perfoliatus Hubei.METHODS:Random amplified polymorphic DNA analysis (RAPD) and experimental animal infection were performed.RESULTS:469 polymorphic DNA fragments were obtained by 28 primers from 4 Echinochasmus species and strains in Fujian,Anhui and Jiangxi.20.8% and 97.6% of the fragments in Echinochasmus fujianensis were the same as those in Echinochasmus japonicus Fujian strain and in Echinochasmus liliputanus Anhui, respectively,99.7% of the fragments were the same between Echinochasmus japonicus Jiangxi strain and Fujian strain. CONCLUSION: Echinochasmus fujianensis and Echinochasmus liliputanus Anhui are the same species.Echinochasmus fujienensis is an independent species different from Echinochasmus japonicus.Polyinfection of Echinochasmus fujianensis,Echinochasmus japonicus and Echinochasmus perfoliatus exist in all the 3 provinces,Hubei,Anbui and Fujian,of which Echinochasmus fujienensis is a dominant species.