人非小细胞肺癌组织中Survivin的表达及与caspase-3和p21WAF1表达的意义

目的:探讨非小细胞肺癌(nonsmallcelllungcancer,NSCLC)组织中survivin、caspase3和p21WAF1的蛋白表达与临床病理指标的关系及survivin与caspase3和p21WAF1蛋白表达之间的关系。方法:在87例NSCLC原发灶中应用免疫组织化学SP法检测survivin、caspase3和p21WAF1蛋白的表达。结果:在87例NSCLC原发灶中survivin、caspase3和p21WAF13种蛋白表达阳性率分别为71.26%、64.37%和48.28%。它们均与组织学类型无关,P>0.05,而与淋巴结转移均显著相关,P<0.05;survivin和caspase3的蛋白表达与肿瘤细胞分化程度无关,P>0.05,但p21WAF1与肿瘤细胞分化程度显著相关,P=0.003。在87例NSCLC中,survivin的表达与caspase3(P=0.001)和p21WAF1均呈显著负相关,P=0.000。结论:Survivin、caspase3和p21WAF1均与NSCLC的浸润进展显著相关。Survivin可能直接作用于caspase3阻断了细胞的凋亡过程,或通过抑制p21WAF1的表达而缩短了细胞周期,从而促进了NSCLC的癌细胞不断增殖。

Expression of survivin protein in human non-small cell lung cancer and its significance between survivin and caspase-3, p21WAF1 proteins

OBJECTIVE:To investigate the expressions of survivin, caspase-3 and p21~ WAF1 proteins in non-small cell lung cancer (NSCLC) and their relationship between their expressions and clinical pathology parameters, and the correlation between survivin protein expression and caspase-3, p21~ WAF1 protein expressions respectively. METHODS: The serial sections of the same tissues were subjected to immunohistochemical stain of survivin, caspase-3 and p21~ WAF1 proteins in 87 patients with NSCLC (42 patients with squamous cell carcinoma, 45 with adenocarcinoma).RESULTS: In 87 NSCLC, survivin, caspase-3 and p21~ WAF1 protein positive rates were 71.26%, 64.37% and 48.28% respectively. The positive expressions of survivin, caspase-3 and p21~ WAF1 proteins were not associated with histology types (P>0.05), but were highly associated with lymph node metastasis (P<0.05); the expressions of survivin and caspase-3 proteins were not associated with cellular differentiation ( P>0.05), but p21~ WAF1 protein was closely associated with cellular differentiation (P=0.003). In 87 NSCLC, a negative correlation was present between survivin and caspase-3 protein expressions (P=0.001), survivin and p21~ WAF1 protein expressions (P=0.000).CONCLUSIONS: There is a significant correlation between survivin, caspase-3 and p21~ WAF1 genes and the infiltration depth, stage in NSCLC. Survivin blocks cell apoptosis by inhibiting caspase-3 activity or shortens cell cycle by inhibiting p21~ WAF1 gene expression, thereby promots proliferation of cancerous cell of NSCLC.