红景天对大鼠脑缺血再灌注时自由基损伤保护作用的实验研究

目的：探讨红景天对脑缺血再灌注时自由基损伤的保护作用，为脑缺血再灌注损伤的防治提供新的药物。方法：用４ＶＯ法复制大鼠实验模型，分为假手术组（ＳＡＭ）、单纯缺血组（ＩＳ）、缺血再灌注组（ＩＲ）、药物预防后缺血再灌注组（Ｒ＋ＩＲ）、缺血再灌注后药物治疗组（ＩＲ＋Ｒ），分别观察了大鼠脑组织中ＬＰＯ、ＳＯＤ、Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ的变化。结果：①ＩＳ组及ＩＲ组大鼠脑组织中ＬＰＯ含量均显著高于ＳＡＭ组（Ｐ＜０．０５，Ｐ＜０．０１）。ＳＯＤ含量均低于ＳＡＭ组（Ｐ＜０．０５，Ｐ＜０．０１），Ｒ＋ＩＲ组及ＩＲ＋Ｒ组大鼠脑组织中ＬＰＯ含量显著降低（Ｐ＜０．０５），而ＳＯＤ含量却显著升高（Ｐ＜０．０１）。②ＩＲ组大鼠脑组织中Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ活性明显低于ＳＡＭ组（Ｐ＜０．０１），Ｒ＋ＩＲ组及ＩＲ＋Ｒ组较ＩＲ组升高（Ｐ＜０．０５，Ｐ＜０．０１）。结论：红景天对大鼠脑缺血再灌注时自由基损伤具有一定的保护作用。

Study on protection of Rhodiola sachalinensis AR against free radical injury of the ischemia reperfusion rats

Objective:To study the protection of Rhodiola sachalinensis AR against free radical injury of the ischemia reperfusion rats,and to offer a new medicine which may protect the ischemia reperfusion injury in brain.Methods:The experimental rats,which were replicafed with 4VO method,were divided into 5 groups:sample (SAM) group,ischemia (IS) group,ischemia reperfusion (IR) group,Rhodiola sachalinensis plus IR group (R+IR) and IR plus Rhodiola sachalinensis (IR+R) group.The changes of LPO,SOD,Na+K+ATPase in brain tissue of rats were observed.Results:The content of LPO of brain tissue of rats in IS group and in IR group were significantly higher than that in SAM group (P<005,P<001),but in R+IR group and IR+R group,contents of LPO were significantly lower than that in IR group;the content of SOD in IS group and IR group were lower than that in SAM gorup,but in R+IR group and in IR+R group,the content of SOD were higher than that in IR group.The activities of Na+K+ATPase of brain tissue rats in IR group were lower than that in SAM group (P<001),and in R+IR and IR+R groups,the activities of Na+K+ATPase were higher than that in IR group (P<005,P<001).Conclusion:The Rhodiola sachalinensis can protect the brain tissue of ischemia reperfusion rats against free radical injury.