胚胎脊髓对损伤脊髓的修复作用研究

目的探讨不同条件下胚胎脊髓移值修复成鼠急性脊髓损伤(SCI)的能力。方法成鼠胸髓损伤后分别移植孕14d胚胎脊髓(FSC组)、游离正中神经加胚胎脊髓(P＋F组)、带血管蒂正中神经加胚胎脊髓(V＋F组)，术后8周行组织学检查。结果V＋F组胚胎脊髓与受体融合佳，体积增长速度、神经纤维和神经元数目显著高于P＋F、FSC组（P<0.01），细胞分化比较好，突触较成熟，界面区也无明显的胶质增生。结论带血管蒂周围胚胎脊髓联合移植，对FSC的生长发育、对损伤神经元的再生能力均有一定的促进作用。

Study of the therapeutic effect of fetal spinal cord allograft to repair spinal cord injury

Objective To observe the repair effect of fetal spinal cord in different conditions.Method A 5 mm cord defect of the left lateral column was made at T1－ 3 vertebral level, and then the defect was grafted randomly using embryonic day 14 fetal spinal cord (Group FSC), free peripheral nerve and fetal spinal cord (Group P＋ F), or vascularized peripheral nerve and fetal spinal cord (Group V＋ F). 8 weeks after surgery, the survival, differentiation of the grafts, and the ability of repairing host spinal cord were assessed.Result In VPN－ FSC co－ graft group, the fetal implant was intimately fused with the host CNS parenchyma, the volume, nerve fibers and neurons density of the fetal implant were significantly higher than Group FSC and Group P＋ F(P<0.01).The majority of neurons in this group were well differentiated ,well-developed synapses were scattered throughout the neuropil.Conclusion The combination of VPN-FSC could prompt the development of fetal implant and support the regeneration of central injured axons to certain degrees.