| 毛细管电泳法测定人工牛黄中胆酸的含量 |
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| 引用本文： | 郑建新,邹登峰. 毛细管电泳法测定人工牛黄中胆酸的含量[J]. 中国药房, 2006, 17(23): 1817-1818 |
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| 作者姓名： | 郑建新 邹登峰 |
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| 作者单位： | 1. 青海省妇女儿童医院,西宁市,810007
2. 桂林医学院天然药物教研室,桂林市541004 |
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| 摘 要： | 目的:建立以毛细管电泳法测定人工牛黄中胆酸含量的方法。方法:运行缓冲液为30mmol/L十二烷基硫酸钠+40mmol/L磷酸氢二钠溶液(pH=9.0),进样量为50mbar×5s,分离电压为20kV,温度为30℃,检测波长为210nm。结果:胆酸检测浓度在60.6～970.0μg/ml范围内线性关系良好(r=0.9980),平均回收率为98%～102%(RSD<5%)。结论:该方法简捷、快速、准确、重现性好,可用于该药材的质量控制。
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| 关 键 词： | 毛细管电泳法 牛黄 胆酸 含量 |
| 文章编号： | 1001-0408(2006)23-1817-02 |
| 收稿时间： | 2006-11-01 |
| 修稿时间： | 2006-11-16 |
Determination of the Cholalic Acid in Artificial Bezoar by CE |
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| ZHEN Jianxin,ZOU Dengfeng. Determination of the Cholalic Acid in Artificial Bezoar by CE[J]. China Pharmacy, 2006, 17(23): 1817-1818 |
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| Authors: | ZHEN Jianxin ZOU Dengfeng |
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| Abstract: | OBJECTIVE:To establish a capillary electrophoresis(CE)method for the determination of the cholalic acid in artificial bezoar.METHODS:The 30mmol/L sodium dodecyl sulfate plus 40mmol/L dibasic sodium phosphate were used as buffer solution(pH=9.0)with the sample size of 50 mbar×5s at30℃,the detection wavelength was 210nm and the separation voltage was 20kV.RESULTS:The linear range of cholalic acid was 60.6～970.0μg/ml(r=0.9980),the average recovery rate was 98%～102%(RSD<5%).CONCLUSION:The method was simple,rapid,accurate and reproducible for the quality control of artificial bezoar. |
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| Keywords: | Capillary electrophoresis Artificial bezoar Cholalic acid Content |
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