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Experimental cerebral hypoperfusion induces white matter injury and microglial activation in the rat brain
Authors:Email author" target="_blank">Eszter?FarkasEmail author  Gergely?Donka  Rob?A?I?de?Vos  András?Mihály  Ferenc?Bari  Paul?G?M?Luiten
Institution:(1) Department of Anatomy, School of Medicine, University of Szeged, PO Box 427, 6701 Szeged, Hungary;(2) Department of Molecular Neurobiology, University of Groningen, Groningen, The Netherlands;(3) Laboratory for Pathology, Enschede, The Netherlands;(4) Department of Physiology, School of Medicine, University of Szeged, Szeged, Hungary
Abstract:Though cerebral white matter injury is a frequently described phenomenon in aging and dementia, the cause of white matter lesions has not been conclusively determined. Since the lesions are often associated with cerebrovascular risk factors, ischemia emerges as a potential condition for the development of white matter injury. In the present study, we induced experimental cerebral hypoperfusion by permanent, bilateral occlusion of the common carotid arteries of rats (n=6). A sham-operated group served as control (n=6). Thirteen weeks after the onset of occlusion, markers for astrocytes, microglia, and myelin were found to be labeled by means of immunocytochemistry in the corpus callosum, the internal capsule, and the optic tract. The ultrastructural integrity and oligodendrocyte density in the optic tract were investigated by electron microscopy. Quantitative analysis revealed that chronic cerebral hypoperfusion caused mild astrogliosis in the corpus callosum and the internal capsule, while astrocytic disintegration in the optic tract increased by 50%. Further, a ten-fold increase in microglial activation and a nearly doubled oligodendrocyte density were measured in the optic tract of the hypoperfused rats as compared with the controls. Finally, vacuolization and irregular myelin sheaths were observed at the ultrastructural level in the optic tract. In summary, the rat optic tract appears to be particularly vulnerable to ischemia, probably because of the rat brainrsquos angioarchitecture. Since the detected glial changes correspond with those reported in vascular and Alzheimer dementia, this model of cerebral hypoperfusion may serve to characterize the causal relationship between ischemia and white matter damage.
Keywords:Astrocyte  Cerebral hypoperfusion  Microglia  Optic tract  White matter
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