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| 郑州地区汉族正常人群、糖尿病和糖尿病并发冠心病患者芳香酯酶Q192R基因多态性 | |
| 引用本文： | 钱书虹,钱庆文,王书珺,王诗瑾. 郑州地区汉族正常人群、糖尿病和糖尿病并发冠心病患者芳香酯酶Q192R基因多态性[J]. 郑州大学学报(医学版), 2003, 38(2): 225-227 |
| 作者姓名： | 钱书虹 钱庆文 王书珺 王诗瑾 |
| 作者单位： | 1. 郑州大学检验系,郑州,450052 2. 郑州大学第一附属医院内科,郑州,450052 3. 郑州水工机械厂职工医院,郑州,450052 4. 河南省临床检验中心,郑州,450052 |
| 基金项目： | 河南省科技攻关基金资助项目991170 75 |
| 摘 要： | 目的：研究郑州地区汉族正常人群、2型糖尿病（DM）及DM并发冠心病（DM－CAD）患者芳香酯酶（Arylesterase/Paraoxonase,ArE/PON1)192位基因多态性。方法：用比色法测定郑州地区汉族正常人群（61例）、DM（60例）和DM－CAD（67例）患者血清Ar/PON1活性，用聚合酶链式反应分析技术检测ArE/PON1基因192位多态性，用酶法和酶联免疫法分别测定血清胆固醇、高密度脂蛋白胆固醇（HDLc)、氧化型低密度脂蛋白（oxLDL)等指标。结果：郑州地区汉族人群中存在有ArE/PON1192位基因多态性，Q、R基因频率健康人群中分别为0.56与0.44；DM组为0.53与0．47；DM－CAD组为0．38和0．62。DM－CAD组R基因频率高于DM组和对照组。基因型百分比DM－CAD组RR（31％）高于DM组（13％）和对照组（11％）（P＜0．05）。DM与DM－CAD组血清ArE/PON1活性水平低于对照组，以DM－CAD组为最低（P＜0．05）。酶活性的降低与HDLc、HDL2C呈正相关，与oxLDL呈负相关。结论：郑州地区汉族人群存在ArE/PON1 192位基因多态性，并提示R基因是郑州地区汉族人群DM－CAD的危险因素。 |
| 关 键 词： | 郑州地区 汉族正常人群 糖尿病 冠心病 芳香酯酶 Q192R 基因多态性 糖尿病并发症 |
| 修稿时间： | 2002-03-07 |
Relationship between arylesterase/paraoxonase1Q192R gene polymorphism and diabetes mellitus complicated with coronary artery disease in population of Han nationality of Zhengzhou |
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| QIAN Shuhong ),QIAN Qingwen ),WANG Shujun ),WANG Shijin ) ). Relationship between arylesterase/paraoxonase1Q192R gene polymorphism and diabetes mellitus complicated with coronary artery disease in population of Han nationality of Zhengzhou[J]. Journal of Zhengzhou University: Med Sci, 2003, 38(2): 225-227 | |
| Authors: | QIAN Shuhong ) QIAN Qingwen ) WANG Shujun ) WANG Shijin ) ) |
| Affiliation: | QIAN Shuhong 1),QIAN Qingwen 2),WANG Shujun 3),WANG Shijin 4) 1)Department of Clinical Laboratory,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052 2)Department of Internal Medicine,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052 3)The Hospital of Zhengzhou Hydraulic Machinery Factory,Zhengzhou 450052 4)Clinical Laboratory Center of Henan Province,Zhengzhou 450052 |
| Abstract: | Aim:To investigate the relationship between arylesterase/paraoxonase(ArE/PON1) Q192R gene polymorphism and type 2 diabetes mellitus (type 2 DM) complicated with coronary artery disease (CAD) in population of Han nationality in Zhengzhou area.Methods:Serum ArE/PON1 activity and its Q192R gene polymorphism were determined in 60 patients with type 2 DM, 67 DM patients complicated with coronary artery disease(DM CAD),and 61 healthy subjects. ArE/PON1Q192R gene polymorphism were detected by PCR method.Results:The frequencies of the Q allele and R were 0.56 and 0.44 in healthy subjects,0.53 and 0.47 in patients with DM,0.38 and 0.62 in patients with DM CAD,respectively. The R gene frequency in DM CAD group was higher than that in DM group and healthy subjects. The percentage of RR genotype in DM CAD group (31%)was higher than that (13%) in DM group and that (11%)in healthy subjects.Serum ArE/PON1 activity in DM and DM CAD groups were lower than that in healthy subjects and it was the lowest in DM CAD group.There were a positive correlation between ArE/PON1 activity and HDLc,HDL2C,and a negative correlation between ArE/PON1 activity and oxLDL.Conclusion:ArE/PON1Q192R polymorphism exists in Zhengzhou population of Han nationality. It is suggested that R allele might be a risk factor for DM complicated with CAD in Zhengzhou population of Han nationality. |
| Keywords: | arylesterase diabetes mellitus coronary artery disease genepolymorphism |
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