| 非离子表面活性剂逆转肿瘤细胞多药耐药性的机制 |
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| 引用本文： | 武建毅,刘岽,唐亮,谈立松.非离子表面活性剂逆转肿瘤细胞多药耐药性的机制[J].实用医药杂志(山东),2007,24(1):87-88,91. |
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| 作者姓名： | 武建毅 刘岽 唐亮 谈立松 |
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| 作者单位： | 上海市肺科医院免疫治疗科 上海200433 |
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| 摘 要： | 目的探讨非离子表面活性剂聚氧乙烯醚﹑聚乙二醇300﹑曲拉通X100对肺癌耐药细胞A549/DDP(耐顺铂)多药耐药性的逆转及其机制。方法用四氮唑盐(MTT)法进行体外耐药逆转实验,Westernblot检测P-gp(P-糖蛋白)蛋白的表达。结果①合用非离子型表活剂前后,A549/DDP对化疗药物阿霉素、顺铂、丝裂霉素、5-氟尿嘧啶、依托泊甙和长春新碱的敏感性显著增加(各组都为P<0.01);②三种非离子型表面活性剂能抑制P-gp蛋白的表达。结论三种非离子表面活性剂具有逆转A549/DDP耐药性的作用,逆转机制与其抑制P-gp蛋白的表达有关。
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| 关 键 词： | 表面活性剂 肺癌 A549/DDP 多药耐药 P-gp |
| 收稿时间： | 2006-08-03 |
| 修稿时间： | 2006-08-03 |
Study on the reversal of cancer cell's multidrug resistance by non-ionic surfactant and its mechanism |
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| WU Jian-yi, LIU Dong, TANG Liang,et al..Study on the reversal of cancer cell''''s multidrug resistance by non-ionic surfactant and its mechanism[J].Practical Journal of Medicine & Pharmacy,2007,24(1):87-88,91. |
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| Authors: | WU Jian-yi LIU Dong TANG Liang |
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| Institution: | the Central Laboratory |
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| Abstract: | Objective To discuss whether the non-ionic surfactant (CremophorEL, PEG300, TritonX-100)can reverse the miltidrug resistance(MDR) of the lung cancer cell strian A549/DDP or not and their mechanism. Methods The reversing drug resistance was studied by MTT, expression of P-gp was detected by Western blot.Results (1)MTT cytotoxic assay revealed that the sensitivities of A549/DDP cells to chemotherapy drugs were significant changed according to presence or absence of non-ionic surfactant(P<0.01); (2)The three non-ionic surfactant can inhibit expression of p-gp.Conclusion The three non-ionic surfactant can reverse the MDR of the lung cancer cell strian A549/DDP,inhibiting expression of p-gp maybe its mechanism. |
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| Keywords: | A549/DDP P-gp |
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