| HPLC法测定止痢宁片中穿心莲内酯含量 |
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| 引用本文： | 王先友,袁琦.HPLC法测定止痢宁片中穿心莲内酯含量[J].河南大学学报(医学版),2009,28(1). |
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| 作者姓名： | 王先友 袁琦 |
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| 作者单位： | 河南大学药学院,分析教研室,河南,开封,475001 |
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| 摘 要： | 目的:研究用HPLC法测定止痢宁片中穿心莲内酯的含量。方法:色谱条件为:Aglient Zorbax ExtentC18柱(250 mm×4.6 mm,5μm),流动相:甲醇∶水=52∶48,流速:1.0 ml/min,检测波长:225 nm,柱温为室温。结果:穿心莲内酯进样量在0.153μg~2.292μg范围内与峰面积呈良好的线性关系(r=0.9996),平均回收率为99.1%,RSD=1.6%。结论:本法灵敏,准确,简便,重现性好。
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| 关 键 词： | 止痢宁片 穿心莲内酯 HPLC |
Determination of Andrographolide contents in Zhilining Tablets by HPLC |
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| WANG Xian-you,YUAN Qi.Determination of Andrographolide contents in Zhilining Tablets by HPLC[J].Journal of Henan University,2009,28(1). |
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| Authors: | WANG Xian-you YUAN Qi |
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| Abstract: | Objective: To determine the contents of Andrographolide in Zhilining tablets.Methods: The accurate result was attained with Aglient zorbax extent C 18 column(250 mm × 4.6 mm,5 μm),using CH 3 OH∶H 2 O=58∶42 as the mobile phase.The flow rate was 1.0 mL/min and the detection wavelength was 225 nm. Results: Linearity was good(r =0.9996) at the range of 0.1528～2.292 μg.Mean recovery was 99.1% with RSD =1.4%. Conclusion: The method is sensitive,accurate and simple with good repeatability. |
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| Keywords: | HPLC |
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