| 高效液相色谱测定血样中阿魏酸含量 |
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| 引用本文： | 燕福生,刘晓东.高效液相色谱测定血样中阿魏酸含量[J].首都医学院学报,1991,12(1):27-30. |
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| 作者姓名： | 燕福生 刘晓东 |
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| 作者单位： | 首都医学院化学教研室，首都医学院化学教研室，首都医学院化学教研室，医学科学院药物所药厂 |
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| 摘 要： | 高效液相色谱法测定血浆中阿魏酸的含量.使用岛津 LC-4A 型高效液相色谱仪.YWG-G_(18)H_(37)填充柱(25cm×4.6mm i.d).以乙二胺、甲酸调至 pH3～4的60%甲醇混合液作流动相.紫外312nm 检测,用内标法定量,内标物为肉桂酸.测定结果,回收率平均值为94.25%,相对标准偏差2.15%(8次测定).标准曲线呈线性关系.本法简便快速、重现性好.
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| 关 键 词： | 阿魏酸 肉桂酸 高效液相色谱 |
| 收稿时间： | 1990-05-03 |
Detection of Ferulic Acid in Blood Samples by Using High Performance Liquid Chromatography(HPLC) |
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| Yan Fusheng Liu Xiaodong Song Xueying,Departmnet of Chemistry,Capital Institute of Medicine.Detection of Ferulic Acid in Blood Samples by Using High Performance Liquid Chromatography(HPLC)[J].Journal of Capital University of Medical Sciences,1991,12(1):27-30. |
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| Authors: | Yan Fusheng Liu Xiaodong Song Xueying Departmnet of Chemistry Capital Institute of Medicine |
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| Institution: | Departmnet of Chemistry, Capital Institute of Medicine |
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| Abstract: | This paper is about the determination of the plasma ferulic acid concentration by high performance liquid chromatograph (HPLC). A Shimadzu liquid chromatograph LC-4A with a column (25cm×4.6mm) of YWG-C18H37(C18 chemical bonded stationary phase) was used.60% methanol containing ethylendiamine and methanoic acid to a mixing liquid with pH value of 3～4 was chosen as the mobile phase.The detection was at 254nm.The quantity of ferulic acid was determined by the internal standard method with cinnamic acid as a reference.The results showed that the standard deviation was 2.15%(eight determinations),the rate of recovery was 94.25% and the standard curve was well linear. The proposed method is simple,rapid and reproducible. |
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| Keywords: | ferulic acid cinnamic acid high performance liquid chromatography |
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