Identification by message selection of cDNA clones encoding antigens of Schistosoma mansoni |
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| Authors: | J S Cordingley W J Haddow V Nene D W Taylor |
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| Affiliation: | 1. Norwegian Institute of Bioeconomy Research (NIBIO), P.O. Box, Ås 1433, Norway;2. International Centre of Insect Physiology and Ecology (icipe), P.O. Box 30772-00100, Nairobi, Kenya;3. Advanced School of Agriculture, Forestry, Water Resources and Environment, University of Ebolowa, P.O. Box 786, Ebolowa, Cameroon;1. Division of Infectious Diseases, Weill Cornell Medicine, New York, NY, USA;2. Aga Khan Hospital, Mwanza, Tanzania;3. Center for Global Health, Weill Cornell Medicine, New York, NY, USA;5. Department of Cell and Chemical Biology, Leiden University Medical Center, Leiden, the Netherlands;6. Department of Parasitology, Leiden University Medical Center, Leiden, the Netherlands;7. Mwanza Intervention Trials Unit, Mwanza, Tanzania;9. Department of Internal Medicine, Catholic University of Health and Allied Sciences, Mwanza, Tanzania;11. Weill Bugando School of Medicine, Mwanza, Tanzania;1. Department of Pathogen Biology, College of Basic Medical Sciences, Naval Medical University, 800 Xiangyin Road, Shanghai 200433, China;2. College of Naval Medicine, Naval Medical University, 800 Xiangyin Road, Shanghai 200433, China |
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| Abstract: | cDNA clone banks have been constructed in a plasmid vector (pJSC73) using mRNA isolated from adult worms and eggs of Schistosoma mansoni. These clone banks have been screened by message selection (hybrid release translation) and clones containing fragments of genes encoding schistosome antigens have been identified by immunoprecipitation of the corresponding mRNA in vitro translation product. Over 50 clones encoding schistosome antigens have been identified including those of the 100 000, 86 000, 28 000, and 27 000 molecular weight surface antigen precursors. |
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