| RP-HPLC法测定复方氧氟沙星滴眼液中氧氟沙星的含量 |
| |
| 引用本文： | 贾淑杰,张蓉,刘美欣,袁瑞军. RP-HPLC法测定复方氧氟沙星滴眼液中氧氟沙星的含量[J]. 中国药房, 2002, 13(2): 109-110 |
| |
| 作者姓名： | 贾淑杰 张蓉 刘美欣 袁瑞军 |
| |
| 作者单位： | 1. 天津市医药科学研究所,天津市,300070
2. 天津市眼科医院,天津市,300020 |
| |
| 摘 要： | 目的 :应用反相高效液相色谱法测定复方氧氟沙星滴眼液中氧氟沙星的含量。方法 :用外标法 ,以Shim -packODS为固定相 ,枸橼酸 (0 05mol/L) -乙腈 -醋酸铵 (0 5mol/L) -0 3 %磷酸 (78∶22∶1∶3)为流动相 ,检测波长为293nm ,流速为1 0ml/min。结果 :氧氟沙星线性范围为8 12μg～40 6μg(r=0 9996 ,n=5) ,平均回收率为99 29 % ,RSD=0 57 % (n=6)。结论 :本法简便、快速、准确
|
| 关 键 词： | 反相高效液相色谱法 氧氟沙星 滴眼液 含量测定 |
| 文章编号： | 1001-0408(2002)02-0109-02 |
| 修稿时间： | 2001-03-05 |
Determination of the Content of Ofloxacin in Compound Ofloxacin Eye Drops by RP- HPLC |
| |
| JIA Shujie,ZHANG Rong. Determination of the Content of Ofloxacin in Compound Ofloxacin Eye Drops by RP- HPLC[J]. China Pharmacy, 2002, 13(2): 109-110 |
| |
| Authors: | JIA Shujie ZHANG Rong |
| |
| Abstract: | OBJECTIVE:To establish a RP-HPLC method to determine the content of ofloxacin in compound ofloxacin eye drops METHODS:Using Shim-pack ODS as fixed phase,a mixture to citric acid(0 05mol/L)-acetonitrile-ammonium acetate(0 5mol/L)-0 3% phosphoric acid(78∶22∶1∶3)as mobile phase,and detecting wavelength:293nm,flow rate:1 0ml/min RESULTS:The linear range of ofloxacin was 8 12μg～40 6μg(r=0 9 996,n=5) The average recovery was 99 29% and RSD(n=6)was 0 57% CONCLUSION:The method is rapid,simple and accurate |
| |
| Keywords: | RP-HPLC ofloxacin eye drops content determination |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
