伸筋易骨矫形手法对兔膝关节软骨细胞代谢及Sox9表达的影响

[目的]探讨伸筋易骨矫形手法对兔膝关节软骨细胞代谢及Sox9表达的影响。[方法]将膝骨关节炎(KOA)模型兔随机分为3组,治疗组采用伸筋易骨矫形手法治疗,对照组采用关节腔注射玻璃酸钠治疗,模型组不做干预,同条件饲养,取材后分离膝骨关节软骨细胞体外培养,镜下观察并通过四甲基偶氮唑盐比色法(MMT)对比3组软骨细胞增殖情况;通过蛋白免疫印迹分析3组软骨细胞Sox9的表达变化。[结果]镜下观察治疗组、对照组关节软骨的增殖速度均快于空白组,其中治疗组增殖速度最快,MMT法检测治疗组软骨细胞增殖能力高于对照组(P0.05),蛋白免疫印迹分析显示治疗组Sox9表达高于对照组(P0.05)。[结论]伸筋易骨矫形手法具有提高软骨细胞增殖速度,增强软骨细胞增殖能力,同时该手法可以提高损伤软骨组织Sox9的表达水平,从而促进软骨细胞的合成代谢。

Effects of Shenjin Yigu orthopaedic manipulation on chondrocytes metabolismand the expression of Sox9 in rabbits

[Objective] To study the effects of Shenjin Yigu orthopaedic manipulation on rabbit chondrocytes metabolism and the expression of Sox9.[Methods] KOA rabbits were randomly divided into 2 groups.The treatment group used Shenjin Yigu orthopaedic manipulation therapy, the control group using intra-articular injection of sodium hyaluronate, culturing chondrocytes in vitro after drawing materials.Microscope observation and comparing of chondrocytes' proliferation between two groups by MMT method, analyzing the expression of Sox9 of chondrocytes between two group by Western-blot method.[Results] Microscopic observation of the treatment group and control group of chondrocytes proliferation faster than those in the blank group and the multiplicaion rate of the treatment group was fastest than those in the control and blank groups, and better proliferation of chondrocytes in the treatment group than that in the control group detected by MMT method(P<0.05).Western blot analysis showed that the expression of Sox9 was higher in the treatment group than that in the control group(P<0.05).[Conclusion] Shenjin Yigu orthopaedic manipulation can improve the speed of proliferation of chondrocytes, enhancing chondrocytes' multiplication ability.Simultaneously this manipulation can increase the expression level of Sox9 in injured cartilage tissue, to promote the synthesis of chondrocyte metabolism ability.