雌激素对雌性大鼠脂质代谢及血糖影响的研究

目的:探讨雌激素对SD大鼠脂质代谢及血糖的影响?方法:将40只7周龄的雌性SD大鼠随机分为4组:①对照组(Con组);②假手术组(sham组);③去卵巢组(OVX组);④去卵巢+雌激素替代组(OVX+E2 组)?术后定期记录体重,给予相应处理,20周龄时测量空腹血糖并进行糖耐量试验,心脏穿刺取血,检测血浆总胆固醇(TC)?甘油三酯(TG)?低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C),同时取腹部脂肪称重?结果:与对照组相比,OVX组大鼠体重明显增加,腹部脂肪重量增加,血浆中TC?TG和LDL-C含量明显增加,空腹血糖无异常改变,但存在一定程度的糖耐量异常?OVX+E2 组使用剂量雌激素替代治疗后,与对照组相比,体重明显减轻,腹部脂肪重量减轻,血脂无异常改变?结论:雌激素水平与体重呈负相关,雌激素水平下降可引起大鼠脂质代谢异常及胰岛素抵抗,补充雌激素后上述异常得到改善?

Effects of estrogen on lipid metabolism and glucose of female rats

Objective:To investigate the effect of estrogen on lipid metabolism and glucose. Methods:Fotry 7-week-old female Sprague-Dawley rats were randomly divided into 4 groups,the control group(Con),sham-operated group(sham),ovariectomy(OVX)group and OVX rats given high dose E2 treatment (OVX+ E2). After being weighted,each group of rats were given corresponding processing. Nine weeks later,the change of visceral fat weight,total cholesterol(TC),triglyceride(TG),low density lipoprotein(LDL),high-density lipoprotein(HDL) and glucose were measured. Results:Compared with the control group,the body weight and abdominal fat of OVX group increased significantly. There was a significant difference in the level of TG,TC and LDL between the control and OVX groups. But in the high dose E2-treated OVX group it showed no significant differences. Compared with sham operation group rats,fasting blood glucose was of no difference in OVX group,but existed impairing glucose tolerance. Conclusion:The estrogen level was negatively correlated with the body weight,lower estrogen induced abnormal lipid metabolism and insulin resistance. But this phenomenon can be reversed after treated with estrogen.