Interaction between dendritic cells and nerve fibres in lymphoid organs after oral scrapie exposure |
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Authors: | Gauthier Dorban Valérie Defaweux Caroline Demonceau Sylvain Flandroy Pierre-Bernard Van Lerberghe Nandini Falisse-Poirrier Joëlle Piret Ernst Heinen Nadine Antoine |
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Institution: | 1. Human Histology, Immunology Center, Faculty of Medicine, University of Liège, CHU, Avenue de l’h?pital, Tour de pharmacie +4, 4000, Liège, Belgium 3. Human Histology, Prion Protein Research Center, University of Liège, Avenue de l’h?pital, CHU Sart-Tilman, Tour de pharmacie +4, 4000, Liège, Belgium 2. Animal Histology, Department of Morphology–Pathology, Faculty of Veterinary Medicine, University of Liège, Boulevard de Colonster, B43a, 4000, Liège, Belgium
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Abstract: | In transmissible spongiform encephalopathies (TSEs), the infectious agent, called PrPsc, an abnormal isoform of the cellular
prion protein, accumulates and replicates in lymphoid organs before affecting the nervous system. To clarify the cellular
requirements for the neuroinvasion of the scrapie agent from the lymphoid organs to the central nervous system, we have studied,
by confocal microscopy, the innervations within Peyer’s patches, mesenteric lymph nodes and the spleen of mice in physiological
conditions and after oral exposure to prion. Contacts between nerve fibres and PrPsc-associated cells, dendritic cells (DCs)
and follicular dendritic cells (FDCs), were evaluated in preclinical prion-infected mice. Using a double immunolabelling strategy,
we demonstrated the lack of innervation of PrPsc-accumulating cells (FDCs). Contacts between nerve fibers and PrPsc-propagating
cells (DCs) were detected in T-cell zones and cell-trafficking areas. This supports, for the first time, the possible implication
of dendritic cells in the prion neuroinvasion process. |
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Keywords: | Prion Dendritic cells Innervation |
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