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| 桑白皮多糖提取工艺正交试验优化研究 | |
| 引用本文： | 何洁英,王汝上,何洁宝,邓小霞.桑白皮多糖提取工艺正交试验优化研究[J].中国中医药信息杂志,2012,19(10):46-47. |
| 作者姓名： | 何洁英 王汝上 何洁宝 邓小霞 |
| 作者单位： | 1. 佛山市第一人民医院,广东,佛山,528000 2. 广州康臣药业有限公司肾病药物研究中心,广东,广州,510530 3. 佛山市顺德区第一人民医院,广东,佛山,528300 4. 广东省第二中医院,广东,广州,510000 |
| 摘 要： | 目的优选桑白皮多糖最佳提取工艺。方法采用正交试验设计,以提取次数、提取时间及醇沉浓度作为工艺因素,每个因素采用3个水平,选择L9（34）正交表进行试验,同时用硫酸-苯酚法测定桑白皮多糖的含量作为评价指标。结果桑白皮多糖的最佳提取工艺为超声提取30 min,提取2次,醇沉浓度为80%。结论优选的工艺多糖提取率稳定,提取率高,提取工艺合理可行。 |
| 关 键 词： | 桑白皮多糖 正交试验 提取工艺 含量测定 |
Optimization of Extraction Process for Polysaccharides in Cortex Mori with Orthogonal Design |
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| HE Jie-ying , WANG Ru-shang , HE Jie-bao , DENG Xiao-xia.Optimization of Extraction Process for Polysaccharides in Cortex Mori with Orthogonal Design[J].Chinese Journal of Information on Traditional Chinese Medicine,2012,19(10):46-47. | |
| Authors: | HE Jie-ying WANG Ru-shang HE Jie-bao DENG Xiao-xia |
| Institution: | 1.The First People’s Hospital of Foshan,Foshan 528000,China;2.The R&D Center of Drug for Renal Diseases,Guangzhou Consun Pharmaceutical Co.,Ltd,Guangzhou 510530,China;3.The First People’s Hospital of Shunde District,Foshan 528300,China;4.Guangdong Second Provincial Hospital of TCM,Guangzhou 510000,China) |
| Abstract: | Objective To optimize the extraction process of polysaccharides of Cortex Mori.Methods Orthogonal experimental design was adopted,with extraction time,ultrasonic time and the concentration of alcohol as factors,3 levels in each factor,and L9(34) orthogonal table was used.The content of Cortex Mori polysaccharides was determined by sulfuric acid-phenol method as the index.Results The optimum extract process was as follows:ultrasonic extracted 30 min for two times,the concentration of alcohol was 80%.Conclusion This method is stable and efficient,the optimum process is reasonable and feasible. |
| Keywords: | Cortex Mori polysaccharides orthogonal design extraction process determination |
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